- Item Description
- Technical Information
- Sample Size: up to 2×108yeast and other fungus species
- Expectant Yield: up to 15µg of aliquot of purified genomic DNA
- Operation Time: 30min or less
- Elution Volume: 50-100µl
- Free sample available upon request
The gYEAST Genomic DNA Reagent Kit has been optimized to provide superior yield and purity. The kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA from Saccharomyces cerevisiae and a variety of other fungus species. This highly versatile solution based system can be scaled proportionately in order to satisfy larger yeast sample sizes, providing a convenient procedure with minimal hands-on time. The provided sorbitol buffer, when combined with β-mercaptoethanol, zymolyase or lyticase will efficiently lyse fungus cell walls consisting of chitin or polysaccharides. The extracted DNA is suitable for use in PCR or other enzymatic reactions.
The gYEAST Genomic DNA Kit is tested on a lot-to-lot basis. Saccharomyces cerevisiae (2x108) is harvested by centrifugation at 5,000 x g for 10 minutes. A 15 µl aliquot of purified genomic DNA from a 100 µl eluate is analyzed by electrophoresis on a 1% agarose gel.
|Specifications||gYeast Mini DNA Kit|
|Sample Type:||Variety of yeast and fungus species|
|Sample Concentration:||2 x 108yeast cells|
|Enzyme:||Lyticase or Zymolyase|
|Typical Yield:||10-15 µg from 2 x 108S. cerevisiae cells|
|Elution Volume:||50-100 µl|
|Operation Time:||<40 minutes|
Does this reagent kit isolate genomic, mitochondrial, and viral DNA simultaneously?Genomic is the most common and readily available DNA hence the name of the kit. However, the kit co-purifies the 3 types of DNA simultaneously. It is difficult to extract mitochondrial DNA so isolating all DNA initially is an acceptable procedure, followed by DNA separation using ultra-centrifuge Cs-Cl gradient if mtDNA is required. Normally only the gDNA and mtDNA in the Yeast cells can be simultaneously extracted using the gYeast Kit unless the yeast cells were infected by a virus, then virus DNA would also be extracted.
Which kit is better for fungi samples?The gYeast kit is the better option for fungi samples versus the gDNA plant kits or the gDNA tissue kit. The gYeast kit includes sorbitol buffer for better extraction.