96 Well Plasmid Kit
Model Number IB47153
- Item Description
- Specifications
- Protocol
- 2x 96 Well Plasmid Kit
- Centrifuge or vacuum format
- 1 – 5 ml cultured bacterial cells
- Filter plates, deep well plates, and collection plates included
The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or a vacuum manifold capable of running 96 well plates. IBI offers IB47500 96 well vacuum manifold.
Typical yields are 20 – 35 µg for high-copy number plasmid and 3 – 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions. Click on the tabs above to review the protocol or detailed specifications.
| Specifications | 96-Well Plasmid Kit | Component | 2 x 96 Well | |
| Format: | 96-Well Plate | PD1 Buffer1: | 40 ml | |
| Binding Capacity: | 35 µg | PD2 Buffer2: | 40 ml | |
| Culture Input: | 1-5 ml | PD3 Buffer: | 60 ml | |
| Culture Type: | Bacteria culture | W1 Buffer: | 80 ml | |
| Plasmid Size: | 1-15 kb | Wash Buffer: | 25 ml | |
| Typical Yield: | 20-35 µg/ well | Elution Buffer: | 30 ml | |
| Elution Volume: | 100 µl | RNase A: (50 mg/ml) | 130 µl | |
| Operation Time: | 25 minutes | Plasmid 96 Well Filter Plate: | 2 (IB47150) | |
| Plasmid 96 Well Binding Plate: | 2 | |||
| 96 Deep Well Plate: | 2 (IB47153) | |||
| 0.35 ml Collection Plate: | 2 | |||
| Adhesive Film: | 8 |
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