• 4 x 96 Well Plasmid Kit
• Centrifuge or vacuum format
• 1 – 5 ml cultured bacterial cells
• Filter plates, deep well plates, and collection plates included

The 4 x 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or avacuum manifold capable of running 96 well plates. IBI offers IB47500 96 well vacuum manifold.

Typical yields are 20 – 35 µg for high-copy number plasmid and 3 – 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions. Click on the tabs above to review the protocol or detailed specifications.

96 Well Plasmid Kit - Protocol

• 10x 96 Well Plasmid Kit
• Centrifuge or vacuum format
• 1 – 5 ml cultured bacterial cells
• Filter plates, deep well plates, and collection plates included

The 10 x 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1 – 5ml of cultured bacterial cells per well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge orvacuum manifold capable of running 96 well plates. IBI offers IB47500 96 well vacuum manifold.

Typical yields are 20 – 35ug for high-copy number plasmid and 3 – 10ug for low-copy number plasmid from 4ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions. Click on the tabs above to review the protocol or detailed specifications.

96 Well Plasmid Kit - Protocol