96 Well Genomic DNA Bacteria Kit

Model Number IB47295


The 96 Well Genomic DNA Bacteria Kit is optimized for high-through put genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

  • Additional Item Description
  • Specifications
  • Protocol

Key Product Details:

The quality of the 96 Well Genomic DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli(5 x 108) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel. The kit also includes:

  • High-throughput genomic and viral DNA extraction from Gram (+) positive and Gram (-) negative bacterial cells
  • Yields up to 5 µg of highly pure genomic DNA per well
  • 96 Well gDNA Binding Plates contain a glass fiber membrane optimized for genomic DNA extraction and sterilized to remove bacteria contamination
  • Includes a Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
  • High-throughput, high yield, high purity genomic and viral DNA extraction from bacteria

Product Specifications:

Sample: up to 5 x 108Gram (+) positive and Gram (-) negative bacteria cells

DNA Size: 20 – 40 Kb

Yield: up to 5 µg of genomic DNA per well

Format: 96 Well gDNA Binding Plate (glass fiber membrane)

Operation Time: within 90 minutes

Elution Volume: 100 - 200 µl

Purity: A260/A280 > 1.9

Kit Storage: dry at room temperature (15 – 25°C); Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

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