- Item Description
High-throughput, high yield, high purity genomic and viral DNA extraction from bacteria
- High-throughput genomic and viral DNA extraction from Gram (+) positive and Gram (-) negative bacterial cells
- Yields up to 5 µg of highly pure genomic DNA per well
- 96 Well gDNA Binding Plates contain a glass fiber membrane optimized for genomic DNA extraction and sterilized to remove bacteria contamination
- Includes a Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
The 96 Well Genomic DNA Bacteria Kit is optimized for high-throughput genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.
The quality of the 96 Well Genomic DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA fromEscherichia coli(5 x 108) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.
Sample: up to 5 x 108Gram (+) positive and Gram (-) negative bacteria cells
DNA Size: 20 – 40 Kb
Yield: up to 5 µg of genomic DNA per well
Format: 96 Well gDNA Binding Plate (glass fiber membrane)
Operation Time: within 90 minutes
Elution Volume: 100 - 200 µl
Purity: A260/A280 > 1.9
Kit Storage: dry at room temperature (15 – 25°C); Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods