- Item Description
- Technical Information
- Sample size: up to 100µl of PCR products per well
- Fragment size: 70bp – 20Kb
- Expectant yield: up to 95%
- Operation time: 20 minutes
The 96 Well PCR Clean Up Kit is designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions using an efficient 96-well binding plate system. Chaotropic salt is used to denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix in each well of the binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution buffer, TE, or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
The quality of the 96 Well PCR Clean Up Kit is tested on a lot-to-lot basis by purifying DNA fragments of various sizes from PCR products. The purified DNA is analyzed by electrophoresis.
What are the comparable competitor kits?Qiagen MinElute 96 UF PCR Purification Kit
I have SDS in my sample; will this adversely affect my gel extraction process?A small amount of SDS in the sample will not adversely affect the gel extraction recovery.