X-Gal

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X-Gal

Model Number IB02264

$29.36

  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS
  • Technical Information

CAS# 7240-90-6

  • Used as a histochemical substrate for β-galactosidase which allows the detection of lac gene expression

X-GAL is a histochemical substrate for β-galactosidase which allows the detection of lac gene expression.

To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20 mg/ml solution. Use either a glass or polypropylene tube. Add 4 ml of this solution per liter of culture media. The tube containing the solution should be wrapped in aluminum foil to prevent damage from light and stored at -20⁰C.

CAS# 7240-90-6
Molecular Formula C14H15BrClNO6
Molecular Weight 408.64
Purity Min. 99%
Solubility (2%, in N,N’ DMF) Clear and colorless
Molecular Biology Specifications
Optical rotation (C=1) - Pass

IB02264 - Specification Sheet

Recommended Use A histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20 mg/ml solution. Use either a glass or polypropylene tube. Add 4 ml of this solution per liter of culture media. The tube containing the solution should be wrapped in aluminum foil to prevent damage by light and should be stored at –20°C. (It is not necessary to sterilize X-GAL solutions by filtration.)

Storage Store desiccated at –20°C. Warm to room temperature before opening. Keep tightly sealed.

What can X-Gal be dissolved in or re-suspended in? Dimethylformamide (DMF) and Dimethyl sulfoxide (DMSO)

How long can this be out of the refrigerator? Not more than 4-6 hours. *If solution turns pink, discard.

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