Trypto Soy Broth

IBI Tryptic Soy Broth Powder – Soybean-Casein Digest Medium for Microbial Cultivation

IBI Tryptic Soy Broth (TSB) Powder, also known as Soybean-Casein Digest Broth or Trypto Soy Broth, is a high-quality dry culture medium designed for the preparation of a liquid microbiological medium. It supports the cultivation of a broad range of microorganisms, including aerobes, anaerobes, and fungi, making it an ideal choice for microbiology labs, pharmaceutical testing, and quality control procedures.

The formulation combines Casein Peptone and Soy Peptone, which deliver essential amino acids, nitrogen, and growth factors to promote robust microbial growth—even for many fastidious organisms—without requiring added blood or serum. Sodium Chloride maintains osmotic equilibrium, Dextrose acts as the fermentable carbohydrate, and Dipotassium Phosphate functions as a pH buffer to stabilize the medium during incubation.

Preparation Instructions for IBI TSB Powder Medium

To prepare the medium, mix 30 grams of IBI Tryptic Soy Broth powder in 1 liter of purified water until evenly dispersed. Heat with continuous stirring and boil for one minute to fully dissolve the powder. Distribute the solution as needed and autoclave at 121°C for 15 minutes to ensure sterility.

IBI’s dry TSB offers reliable performance and batch-to-batch consistency, making it an essential component in microbiological workflows focused on microbial growth, sterility testing, and media validation.