- Item Description
- Retention of unincorporated NTPs: up to 99%
- Recovery of DNA (˃72bp): ˃90%
- Operation time: up to 10 minutes
- DNase- and RNase-free
The Nu-Clean R50/Sephadex G-50 spin columns are intended for use in desalting and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from end-label, fill-in, nick-translation, or random primed DNA labeling reactions. This column should be used to purify DNA greater than 72 base pairs in length or RNA greater than 72 base length; smaller DNAs or RNAs will be retained in the column material. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.
Each DNase- and RNase-free column is prepackaged with Sephadex G-50 in sterile STE buffer. Two autoclavable collection tubes and one non-autoclavable cap are supplied for each column.This product requires a swing bucket centrifuge rotor.
The optimal sample loading volume is 50µl (100µl Maximum) with a maximum of 100µg of nucleic acid per column.
Sample Size:Up to 100ul
Format:Gel filtration in spin column
G50 Sephadex inside spin column containing frit filter. Column capped on both ends.