Nu-Clean R50/Sephadex, G-50, STE

Model Number IB06080

  • Item Description
  • Specifications
  • Protocol
  • Retention of unincorporated NTPs: up to 99%
  • Recovery of DNA (˃72bp): ˃90%
  • Operation time: up to 10 minutes
  • DNase- and RNase-free

The Nu-Clean R50/Sephadex G-50 spin columns are intended for use in desalting and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from end-label, fill-in, nick-translation, or random primed DNA labeling reactions. This column should be used to purify DNA greater than 72 base pairs in length or RNA greater than 72 base length; smaller DNAs or RNAs will be retained in the column material. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume. 

Each DNase- and RNase-free column is prepackaged with Sephadex G-50 in sterile STE buffer. Two autoclavable collection tubes and one non-autoclavable cap are supplied for each column.This product requires a swing bucket centrifuge rotor.

The optimal sample loading volume is 50µl (100µl Maximum) with a maximum of 100µg of nucleic acid per column.

Sample Size:Up to 100ul
Format:Gel filtration in spin column
Carrier Buffer:STE
G50 Sephadex inside spin column containing frit filter. Column capped on both ends.

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