- Item Description
- Technical Information
- Replacement Columns and Collection Tubes
- Replacement Buffers
- Sample Size: 50-200µl whole human blood/up to 5×106cultured mammalian cells/1×109cultured bacterial cells
- Expectant Yield: up to 5-30µg
- Operation Time: 30min or less
- Elution Volume: 50µl
- Free sample available upon request
The Mini Total RNA Sample Kit for blood and cultured cells is specially designed for purification of total RNA from fresh whole human blood and cultured cells. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-Free water. The entire procedure can be completed within 30 minutes. The purified RNA is ready for RT-PCR, Northern Blotting, Primer Extension, and cDNA library construction.
The quality of the Total RNA mini Kit for blood and cultured cells is tested on a lot-to-lot basis by isolating total RNA from blood and cultured cell samples. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.
|Mini Total RNA Kit (Blood & Cultured Cells)
|300 µl of Whole blood 5 x 106Cultured animal cells
|Blood and Cells
|2-3 µg from 300 µl Blood 25-30 µg from 5 x 106Cells
Can the Total RNA replacement columns be used for miRNA application?No, there are subtle differences between the Total RNA and miRNA columns.
What is the minimum sample size of mammalian cells that can be run in the Total RNA Blood & Cultured cell kit?1 x 105of cultured mammalian cells would be the minimum sample size. 5 x 106is the maximum sample size for mammalian cells.