- Item Description
- Retention of unincorporated NTPs: ˃95%
- Recovery of DNA (˃72bp): ˃90%
- Recovery of RNA (˃72 bases): ˃80%
- Operation time: up to 10 minutes
- DNase- and RNase-free
The Midi Select-D, G-50 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>72bp, smaller DNAs will be retained), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5’ end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Midi Select-D G-50 spin columns are RNase-free and can be used for the rapid purification of RNA (>72 bases) away from unincorporated ribonucleotides (rNTPs) and in other related applications. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.
Each DNase- and RNase-free column is prepackaged with Sephadex G-50 in sterile TE buffer. Two nuclease-free collection tubes (autoclavable) are supplied for each column.
The optimal sample loading volume is 50µl with a maximum of 50µg of nucleic acid per column.
Sample Size:Up to 50ul
Format:Gel filtration in spin column
G50 Sephadex inside spin column containing frit filter. Column capped on both ends.