- Item Description
- Retention of unincorporated NTPs: up to 90%
- Operation time: up to 10 minutes
- DNase- and RNase-free
The Midi Select-D TE, G-25 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>12mer), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5’ end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Midi Select-D TE, G-25 spin columns are RNase-free and can be used for rapid purification of oligoribonucleotides and RNA from unincorporated ribonucleotides (rNTPs) and in other related applications. After a brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.
Each DNase- and RNase-free column is prepackaged with Sephadex G-25 in sterile TE buffer. Two nuclease-free collection tubes (autoclavable) are supplied for each column.
Sample Size:Up to 50ul
Format:Gel filtration in spin column
G25 Sephadex inside spin column containing frit filter. Column capped on both ends.