LB Lennox Broth

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LB Lennox Broth

Model Number IB49111

$56.16
  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS
  • Technical Information
  • Contains casein digest peptone, sodium chloride, and yeast extract
  • For maintaining and propagatingE.coliculture

IBI’s LB Lennox Brothis used in molecular genetic studies. It is a nutritionally rich media for the growth and maintenance of pure cultures of recombinant strain ofE. coli. The inclusion of casein peptone and yeast extract supplies essential growth factors to theE. colibacterial culture; such as nitrogen, carbon, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes.

To prepare, mix 20 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store at room temperature, tightly sealed, and protect from moisture and light.

Physical Specifications
CAS#: N/A
pH (@ 25°C): 7.0 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications
Escherichia coli ATCC 23724: Growth
Escherichia coli ATCC 33694: Growth
Escherichia coli ATCC 33849: Growth
Escherichia coli ATCC 39403: Growth
Escherichia coli ATCC 47014: Growth
Escherichia coli ATCC 53868: Growth

LB Lennox Broth Specification Sheet

Formula per Liter
● Casein Digest Peptone 10.0 gm
● Sodium Chloride 5.0 gm
● Yeast Extract 5.0 gm

Preparation
● Mix 20 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use
IBI’s LB Lennox Broth is used in molecular genetic studies. It is a nutritionally rich media for the growth and maintenance of pure cultures of recombinant strains of E.coli. The inclusion of casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins. Sodium chloride also provides essential electrolytes.

Storage
Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.

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