- Item Description
- Technical Information
- Sample Size: up to 250mg agarose gel/up to 100µl PCR products
- Fragment Size: 100bp – 50Kb
- Binding Capacity: 5µg DNA per 10µl of silica beads
- Expectant Yield: 100bp – 10Kb=70-95%, >10Kb=60-80%
- Operation Time: 20min for PCR Cleanup/30min for Gel extraction
- Free sample available upon request
The Large DNA Fragment Extraction Kit was designed to recover or concentrate a broad range of DNA fragments (100bp – 50Kb) from agarose gel, PCR, or other enzymatic reactions. A concentrated sodium buffer is used to dissolve agarose gel and denature enzymes. DNA fragments are then bound by silica beads. Contaminants are removed with Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE Buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. 10 - 20µl of Elution Buffer is used to obtain a high concentration of purified DNA which is ready for use in downstream applications.
The Large DNA Fragment Extraction Kit includes pH indicator for easy determination of optimal pH and sodium acetate to adjust pH if it becomes too high following gel dissociation or PCR product reaction.
The quality of the Large DNA Fragments Extraction Kit is tested on a lot-to-lot basis by purifying DNA fragments from agarose gel, PCR products, or other aqueous solutions and analyzed by electrophoresis.
Sample Size:up to 250mg of agarose gel / up to 100ul of PCR product.
Format:Modified Silica bead and Spin column
Max Fragment size:100bp – 50kb
Expected yield:100bp to 10kb = 70 – 95%, >10kb = 60 – 80%
Elution volume:10 – 20ul
Operation time:20 minutes for PCR Clean-up and 30 minutes for gel extraction
Applications:PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling, Ligation.
What is the competition’s comparable kit?QIAGEN QIAEX II Gel Extraction Kit
I have SDS in my sample; will this adversely affect my gel extraction recovery?A small amount of SDS will not adversely affect the gel extraction recovery.