For research use only. Not for human, veterinary, or therapeutic use. Sold as a laboratory research kit. High-throughput 96-well isolation of total RNA from cultured cells, tissue and bacteria for in-vitro analysis.
The IBI Scientific Isolate 96-well Total RNA Purification kit is based on a phenol and guanidine isothiocyanate solution coupled with a 96-well binding plate system for high throughput purification of high-quality Total RNA from a variety of samples. This system provides maximum Total RNA extraction from difficult samples high in polysaccharides and lipids. The binding plate and wash procedures ensure high purity of eluted RNA.
- Instructions
- Additional Item Description
- Specifications
- Protocol
- Parts & Accessories
How To Use:
To begin, samples are homogenized in IBI Scientific Isolate reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization simply bind, wash and elute the high-quality Total RNA in RNase Free water and use in a variety of sensitive downstream applications. Process is completed in as little as 60 minutes.
Additional Key Details:
- 2 x 96 well Isolate RNA kit, 4 x 96 well Isolate RNA kit or 10 x 96 well Isolate RNA kit.
- Vacuum or Centrifuge format
- Sample: Up to 2 x 10 6 cultured cells, 10 – 20 mg of animal tissue, up to 150 µl of liquid samples (animal whole blood, serum or plasma), up to 1 x 10 9 bacteria cells, 10 – 20 mg of plant tissue.
- Binding Plates, Collection Plates and Deep well plates included.
- DNAse 1 reagent kit and Bacteria Lysis reagent kit (Lysozyme) sold separately.
IB47635 – 2 x 96-Well Total RNA Pure Kit
IB47636 - 4 x 96-Well Total RNA Pure Kit
IB47637 - 10 x 96-Well Total RNA Pure Kit
Applications: Animal tissue, adherent cultured cells, suspension cultured cells, plant tissue, bacteria, and animal research samples including whole blood, serum and plasma.
Specifications:
- Sample: up to 2 x 106 cultured cells, 10-20mg of animal tissue, up to 150 µl of liquid samples (animal whole blood, serum or plasma), up to 1 x 109 bacteria cells, 10-20 mg of plant tissue per well
- Binding Capacity: 50 μg of RNA per well
- Format: Isolate Reagent combined with RNA 96 Well Binding Plate
- Operation Time: 60 minutes
- Elution Volume: 60-80 μl (dead volume: 20-25 μl)
- Kit Storage: dry at room temperature (15-25C)

