RNA Pure Kit

$27.58

Item Description
Product Video
Specifications
Protocol
Technical Information

Sample Size: 50 μg of total RNA
Operation time: 10 minutes
Recovery: Up to 80% of high quality RNA
Elution Volume: 20 - 50 μl
Compatibility: Purify RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using IBI Isolate
TRI-Reagent®, TRIzol®, RNAzol® and QIAzol® etc.
Applications: RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay
Additional Requirements: 1.5 ml microcentrifuge tubes, absolute ethanol

The RNA Pure Kit uses a simple and efficient spin column procedure to purify Total RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using acid-guanidinium-phenol-chlorofom based methods such as TRIzol® Reagent and IBI Isolate. Contaminants such as RNases, DNA and residual phenol are effectively removed using a simple 4 step procedure. The high-quality, total RNA is eluted in RNase-free Water or TE (RNase-free) and is ready for use in a variety of sensitive downstream applications.

For more details click on Protocol or Technical Information below.

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RNA Pure Kit Protoco l

The extracted RNA can be used directly in a variety of downstream applications such as RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay.

Quality Control

The RNA Pure Kit is tested on a lot-to-lot basis. Following RNA purification using the RNA Pure Kit, 10 μl from a 50 μl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Item Description
Product Video
Specifications
Protocol
Technical Information

Sample Size: Scalable for blood, buffy coat, serum, plasma, cultured cells, and tissue
Operation Time: up to 60 minutes
Can simultaneously extract DNA, RNA, and protein from the same sample

IBI Isolate is a phenol, chloroform, and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA, and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells, and tissue.

For more details click on Protocol or Technical Information below.

Sample: Adherent Cultured Cells, Suspension Cultured Cells, Body fluids including, Blood, Buffy Coat, Serum, and plasma.
Format: Phenol, chloroform, and guanidine isothiocyanate based scalable solution.
Extract simultaneous RNA-DNA and protein from the same sample using phase separation.

IBI Isolate Protocol

The extracted RNA can be used directly in a variety of downstream applications such as cDNA library construction, cloning, RT-PCR (Endpoint), real-time PCR, nuclease protection assays, and northern blotting.

Quality Control

IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

How does the IBI Isolate DNA/RNA Reagent Sample Kit work? This kit uses a phenol-chloroform and guanidine isothiocyanate solution to simultaneously extract DNA, RNA and protein from a variety of samples. Once the sample is lysed, aqueous layers will form in the tube. The first, clear layer contains RNA, the second, white layer contains DNA & protein, the third, red layer contains organics, DNA and protein.

How do I just retrieve the protein from the White (interphase) and Red phase? Following DNA precipitation, add 100% ethanol into the interphase and organic phase, the proteins are in the phenol-ethanol supernatant. The proteins can be precipitated by adding isopropanol to the phenol-ethanol supernatant.

What kind of RNA yield can we expect from the Isolate Reagent Sample Kit? RNA was extracted from (1 x 10⁶) SSN-1 cells (fish cell) by the IBI Isolate, the concentration of RNA is 500 ng/µl, and the total yield is found to be 25 µg RNA.

Item Description
Product Video
Specifications
Protocol
Technical Information

Sample Size: Scalable for blood, buffy coat, serum, plasma, cultured cells, and tissue
Operation Time: up to 60 minutes
Can simultaneously extract DNA, RNA, and protein from the same sample

IBI Isolate Protocol

Quality Control

How do I just retrieve the protein from the White (inter-phase) and Red phase? Following DNA precipitation, add 100% ethanol into the inter-phase and organic phase, the proteins are in the phenol-ethanol supernatant. The proteins can be precipitated by adding isopropanol to the phenol-ethanol supernatant.

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RNA Pure Kit

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