Model Number IB07200

  • Item Description
  • Physical Specifications
  • Technical Information
  • Certificate of Analysis
  • SDS

  • CAS#  9005-64-5
  • 500ml Bottle of Tween 20 OR 1 liter bottle of TWEEN 20
  • A non-ionic detergent
  • DNase, RNase and Protease free


Tween 20 is a non-ionic detergent widely used in biochemical applications. It can be used as an emulsifying agent to prepare stable oil-in-water emulsions. TWEEN 20 can be used to remove peripheral protein from membranes. TWEEN 20 is commonly used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%.

View/Download Specifications Sheet

Physical Specifications:

Appearance ___________________________________ Yellowish liquid

Hydroxyl Value ________________________________ 96 -108

Saponification Value ____________________________ 40 - 50

Moisture _____________________________________ < 3.0%

Dioxane ______________________________________ < 10ppm

Acid Value ____________________________________ < 2.0

Gardner Color _________________________________ < 5.0

BSE/TSE Free _________________________________ Pass

Country of Origin _______________________________ U.S.A. 

TWEEN 20 is commonly used as a component of:

  • Blocking buffer in immunohistochemistry
  • Washing buffer and blocking buffer in Western Blotting
  • Washing buffer in ELISA (Enzyme Linked Immunosorbent Assay)
  • Reaction mixture in PCR  (Polymerase Chain Reaction)


TWEEN 20 is polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a non-ionic detergent used commonly in biochemical applications. 

TWEEN 20 can be stored at room temp in a cool dry area and the bottle should be sealed tightly when not in use. 

IBI TWEEN 20 can be used as washing agent immunoassays such as Western Blots and ELISAs. It helps prevent non-specific antibody binding. In these applications it is typically dissolved in Tris-Buffered saline (TBS) or Phosphate Buffered saline (PBS) at dilutions of 0.05% in 1X or 0.5% in 10X solution. These buffers are used for washes between each immune-reaction, to remove unbound immunologicals, and eventually for incubating solutions of immuno-reagents such as labeled antibodies in order to reduce nonspecific background. 

For premixed PBS with TWEEN 20 see IBI model numbers IB70167 and IB70168

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