
- Item Description
- Physical Specifications
- Technical Information
- Certificate of Analysis
- SDS
- CAS# 9005-64-5
- 500ml Bottle of Tween 20 OR 1 liter bottle of TWEEN 20
- A non-ionic detergent
- DNase, RNase and Protease free
Tween 20 is a non-ionic detergent widely used in biochemical applications. It can be used as an emulsifying agent to prepare stable oil-in-water emulsions. TWEEN 20 can be used to remove peripheral protein from membranes. TWEEN 20 is commonly used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%.
View/Download Specifications Sheet
Physical Specifications:
Appearance ___________________________________ Yellowish liquid
Hydroxyl Value ________________________________ 96 -108
Saponification Value ____________________________ 40 - 50
Moisture _____________________________________ < 3.0%
Dioxane ______________________________________ < 10ppm
Acid Value ____________________________________ < 2.0
Gardner Color _________________________________ < 5.0
BSE/TSE Free _________________________________ Pass
Country of Origin _______________________________ U.S.A.
TWEEN 20 is commonly used as a component of:
- Blocking buffer in immunohistochemistry
- Washing buffer and blocking buffer in Western Blotting
- Washing buffer in ELISA (Enzyme Linked Immunosorbent Assay)
- Reaction mixture in PCR (Polymerase Chain Reaction)
TWEEN 20 is polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a non-ionic detergent used commonly in biochemical applications.
TWEEN 20 can be stored at room temp in a cool dry area and the bottle should be sealed tightly when not in use.
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