- Item Description
- For use with leftover reagents from IBI MiniPlasmid Kits or competitive products
- Sample Size: 1 – 4ml bacterial culture
- Elution Volume: 50 – 100µl
- Binding Capacity: Up to 30µg
The Replacement Mini Plasmid Columns and Collection Tubes are for use with leftover reagents from IBI Mini Plasmid Kits including the following item numbers: IB47101, IB47102, IB47170, IB47171, and IB47172.
The columns and collection tubes can also be used with comparable, competitive Mini Plasmid Kit reagents that utilize a bind, wash, and elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
Sample Size: 1 – 4ml bacterial culture
Format: Spin column or vacuum manifold
Expected yield: 20 – 30ug for high copy and 3 – 10ug for low copy.
Elution volume: 50ul to 100ul
Operation time: 15 minutes
Purified plasmid DNA is ready for use in ligation, PCR, restriction enzyme digestion, and sequencing reactions.