Replacement DF Columns and Collection Tubes – 25 Pack
- For use with leftover reagents from IBI PCR/Gel Extraction Kits or competitive products
- Sample Size: 300mg agarose gel/100µl PCR product
- Elution Volume: 20 – 50µl
- Binding Capacity: Up to 10µg
The Replacement DF Columns and Collection Tubes are for use with leftover reagents from IBI PCR/Gel Extraction Kits including the following item numbers: IB47020 and IB47030.
These columns and collection tubes can also be used with comparable, competitive PCR/Gel Extraction Kit reagents that utilize a bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.
Sample Size: 300mg of agarose gel or 100µl of PCR product
Format: Spin column or vacuum manifold
Expected Yield: 90% for Gel extraction and 95% for PCR Clean-up
Elution Volume: 20 - 50µl
Operation Time: 20 minutes
Purified DNA is ready for use in Fluorescent or Radioactive sequencing, ligation, PCR, restriction enzyme digestion, and DNA labeling.