Proteinase K Powder

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Proteinase K Powder

Model Number IB05400

$89.33
  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS
  • Technical Information

IBI Scientific Recombinant Proteinase K Powder, originally isolated from the mold Engyodontium album, is a serine protease with broad substrate specificity and relatively high proteolytic activity. It preferentially cleaves ester and peptide bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. 

IBI Scientific recombinant Proteinase K Powder is highly pure, determined by SDS-PAGE to be 95%.

High purity, sterility, no bio-burden and no presence of DNase, RNase, DNA, and RNA contaminants.

By using absorbance A275 as the vertical axis and different concentrations of a tyrosine as the horizontal axis the enzymatic activity was calculated to >30U/mg.

IBI Scientific recombinant Proteinase K Powder is expressed in pichia pastoris, purified and lyophilized. 

Physical Specifications:
Molecular Weight
= 28.9 kda
Enzyme Activity = >30U/mg
DNase Assay = None Detected
RNase Assay = None Detected
Form: Lyophilized Powder

Storage:

  • -20C for up to 2 years
  • 4C for up to 18 months
  • Room temperature for up to 12 months
  • Is best to make aliquots for multiple uses
  • Avoid repeated freeze-thaw cycles

IB05400 - Specification Sheet

To place this product into solution...

  • 50mM Tris
  • 3mM CaCl2
  • 50% Glycerol
  • Adjust pH to 8.0 with either HCl or NaOH
  1. Dissolve solids in 50% volume of Certified Nuclease Free Water (IB42100)
  2. QS to 95% with glycerol. pH to 8.0 using HCL or NaOH.
  3. QS to 100% volume with glycerol.
  4. Recheck pH using different meter.
  5. Mix well using a sterile stir bar and filter through a 0.2 micron sterile filter.
  6. Store buffer at 4C and let chill before preparing Proteinase K solution.

Using cold buffer, remove Proteinase K from freezer and dilute powder to 10mg,mL with buffer. Mix well using a stir bar. Filter solution through a 1.2 micron PES membrane syringe or disc filter and aseptically fill into final container. Aliquot and store at -20C. 

  • Use containers and materials that have low protein binding characteristics. 

Note: If you are able to use this product entirely in 15 days or less, IBI Recombinant Proteinase K Powder can be placed directly into Pure water and 50% glycerin at required concentration. 

 

 

 

 

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