- Item Description
- Technical Information
- Total RNA Replacement Columns and Collection Tubes
- Sample Size: up to 25mg tissue/up to 25mg paraffin-embedded tissue
- Operation Time: 15min or less
- Expected Yield: 5 – 30µg RNA
- Elution Volume: 25-100µl
The Total RNA Mini Kit for Tissue was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase (optional in-column DNase treatments can be followed). RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed , using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. The procedure does not require phenol extraction or alcohol precipitation and can be completed within 15 minutes. The purified RNA is ready-for-use in RT-PCR, northern blotting, primer extension, and cDNA library construction.
The quality of the Total RNA Mini Kit for Tissue is tested on a lot-to-lot basis according to IBI’s ISO-certified quality management system. Total RNA is isolated from a 25 mg animal tissue sample, quantified with a spectrophotometer, and analyzed by electrophoresis.
|Specifications||Mini Total RNA Kit (Tissue)|
|Binding Capacity:||50 µg|
|Sample Concentration:||25 mg of Tissue 25 mg of Paraffin-embedded Tissue|
|Sample Type:||Animal Tissue|
|Typical Yield:||5-30 µg|
|Elution Volume:||50 µl|
|Operation Time:||<15 mins.|
Can the Total RNA replacement columns be used for miRNA application? No, there are subtle differences between the Total RNA and miRNA columns.
Can the RNA Tissue Kit be used for insects? Yes, the RNA Tissue Kits can be used for this process.
Can the Total RNA Tissue kit be used for heart tissue? It should be fine as long as the tissue is completely homogenized and is a fresh sample.