HEPES, Free Acid

Model Number IB01130


HEPES powder is a zwitterionic biological buffer ideally suited for cell culture due to its ability to maintain physiological pH despite changes in carbon dioxide concentration. This property distinguishes HEPES from bicarbonate buffers which are also used in cell culture applications. Another characteristic of HEPES is that its disassociation decreases as temperature decreases making it a more effective buffer agent for maintaining enzyme structure and function at low temperatures.

  • Additional Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS

HEPES Concentrations:       

Typical concentrations range from 10 to 25 mM. HEPES has a buffering range of pH 6.8 to 8.2. HEPES is also a component of Tris-HEPES which is a buffer commonly used in polyacrylamide gel electrophoresis of proteins.                

Offered in 50g, 250g, 500g and 1Kg sizes for your convenience.

Key Details:

  • For use in polyacrylamide electrophoresis buffer systems.         
  • Ideal cell culture buffer.                 
  • Maintains physiological pH in cell culture applications.  
  • Physical Specifications:

    Appearance: White Crystalline Powder
    Molecular Formula:C8H18N2O4S
    Molecular Weight:238.31
    pH (0.5 M solution in H2O):5.1 to 6.5
    Purity:Min. 99.0
    Water:Max. 0.25%
    Heavy Metals: Max. 0.001%
    Iron: Max. 0.0005%
    Sulfates: Max. 0.05%

    Molecular Biology Specifications:

    DNase assay:None Detected
    RNase assay:None Detected
    Protease assay:None Detected
    Cell Culture Testing:Pass

    IB01130 - Specification Sheet

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