- Item Description
- Physical Specifications
- Certificate of Analysis
- For use in polyacrylamide electrophoresis buffer systems.
- Idealcell culture buffer.
- Maintains physiological pH in cell culture applications.
HEPES powderis a zwitterionic biologicalbuffer ideally suited for cell culture due to its ability to maintain physiological pH despite changes in carbon dioxideconcentration. Thisproperty distinguishes HEPES frombicarbonate buffers which are also used in cell culture applications.Another characteristic of HEPES is that its disassociation decreases as temperature decreases making it a more effective buffer agent for maintaining enzyme structure and functionat low temperatures.
Typicalconcentrations range from10 to 25 mM. HEPES has a buffering range of pH 6.8 to 8.2.
HEPES is also a component of Tris-HEPES which is a buffer commonly usedin polyacrylamide gel electrophoresis of proteins.
Offered in 50g, 250g, 500g and 1Kg sizes for your convenience.
Appearance:White Crystalline Powder
pH (0.5 M solution in H2O):5.1 to 6.5
Heavy Metals:Max. 0.001%
Molecular Biology Specifications
DNase assay:None Detected
RNase assay:None Detected
Protease assay:None Detected
Cell Culture Testing:Pass