HEPES, Free Acid

Model Number IB01130

  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS

CAS# 7365-45-9

  • For use in polyacrylamide electrophoresis buffer systems.
  • Idealcell culture buffer.
  • Maintains physiological pH in cell culture applications.

HEPES powderis a zwitterionic biologicalbuffer ideally suited for cell culture due to its ability to maintain physiological pH despite changes in carbon dioxideconcentration. Thisproperty distinguishes HEPES frombicarbonate buffers which are also used in cell culture applications.Another characteristic of HEPES is that its disassociation decreases as temperature decreases making it a more effective buffer agent for maintaining enzyme structure and functionat low temperatures.

Typicalconcentrations range from10 to 25 mM. HEPES has a buffering range of pH 6.8 to 8.2.

HEPES is also a component of Tris-HEPES which is a buffer commonly usedin polyacrylamide gel electrophoresis of proteins.

Offered in 50g, 250g, 500g and 1Kg sizes for your convenience.

Physical Specifications
Appearance:White Crystalline Powder
Molecular Formula:C8H18N2O4S
Molecular Weight:238.31
pH (0.5 M solution in H2O):5.1 to 6.5
Purity:Min. 99.0
Water:Max. 0.25%
Heavy Metals:Max. 0.001%
Iron:Max. 0.0005%
Sulfates:Max. 0.05%

Molecular Biology Specifications
DNase assay:None Detected
RNase assay:None Detected
Protease assay:None Detected
Cell Culture Testing:Pass

IB01130 - Specification Sheet

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