Formamide, Spectral Grade

Model Number IB72024


Spectral Grade Formamide is used as a denaturant in hybridization and is used in hybridization buffers made from standard recipes. Formamide separates the two strands of the double helix. It can also be used as a cryoprotectant and gel stabilizer. Ideal for preparing brain sections of mice for BrdU immunohistochemistry and FISH analysis of HeLa cells.

  • Item Description
  • Specifications
  • Certificate of Analysis
  • SDS

Additional Description:

IBI Formamide is used in hybridization, electrophoresis applications, and to denature DNA and RNA. IBI Formamide is supplied in an amber glass bottle, it is distilled, deionized, and packaged under nitrogen to prevent oxidation. Bottles that have been opened and stored should be deionized prior to use by mixing 1 gm of mixed bed resin per 10 ml of formamide for 30 minutes. It is a clear liquid amide derived from formic acid.

The purity of IBI formamide is determined by way of gas chromatography.

Physical Specifications:

CAS#: 75-12-7
Formula Weight: 45.04
Molecular Formula: CH3NO
Purity: Min. 99.0%
Conductivity: Max. 100 μmho
A280: Max. 0.08
Iron: <0.0005%
Copper: <0.00001%
Lead: <0.00005%
Zinc: <0.00005%

Molecular Biology Specifications:

DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected

IB72020 - Specification Sheet

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