Cell Culture Grade Water

Model Number IB42010

  • Item Description
  • Product Video
  • Specifications
  • Certificate of Analysis
  • SDS

IBI Cell Culture water is ideal for nuclear transfer, transfection, transformation, cell and tissue culture, LAL testing and all other aqueous solution preparations that require stringent quality control.

IBI Cell Culture water is developed using the latest in water purification technology including reverse osmosis, EDI, UV Light and ultra-filtration all built into one large system. The EDI module contains special resins to deionize the water and achieve 18 meg-ohm of resistivity. The water then passes through a 0.2 micron filter before being exposed to a specially tuned UV light frequency that will oxidize all organic material. After UV oxidation the water passes through an ultra-filtration membrane which removes all oxidized organics and renders the bacterial endotoxin levels in the water to less than 0.005 EU/ml. Ultrafiltration also produces Nuclease Free Water, meaning No DNase, RNase or Protease.

Water samples from this system are regularly tested and validated by 2 independent laboratories testing for nuclease activity and bacterial endotoxin load. IBI internal quality control methods are tested and validated against the results of the independent laboratories using same samples. 

IBI cell culture water is bottled in sterile bottles, aseptically, inside an ISO 7 sterile suite inside a fume hood. 

IBI conducts quality control testing for each specific lot of cell culture water and the results are noted on the certificate of analysis. IBI also conducts 14-day USP sterility testing on all cell culture water products.

Specifications For Cell Culture Grade Water

Grade Cell Culture Grade
Cations & Anions Free: Yes
Mycoplasma Free: Yes
Sterility Assurance By: 0.1 µm pore filter
Resistivity: 18.2 mOhms
Endotoxin (LAL): ≤ 0.005 EU/ml
Electrophoresis: Yes
MEA Approved: Yes
RNAse: N/A
DNAse: N/A
Nickase: N/A
Protease: N/A
Phosphatase: None Detected
Mycoplasma: None Detected
Heavy Metals: ≤ 0.00004%
Iron (Fe): Not Tested
Sterility Assured: 0.1 µm sterile-filtered and 2 X autoclaved
Available Bottle Sizes: 1 L, 2 L, 10 L

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