Blue Clean Protein Stain

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Blue Clean Protein Stain

Model Number IB01034

$75.45

  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS
  • Technical Information
  • Less hazardous alternative to Coomassie Blue Stain
  • Exhibits sensitivity below 20ng of protein per band
  • This product ships as a hazardous material

Blue-Clean Protein Stain is a more environmentally friendly alternative to traditional coomassie blue protein staining procedures in SDS-PAGE gels. Blue-Clean Protein Stain is a ready-to-use stain that does not contain methanol or acetic acid or require hazardous solvents for destaining. The simple hands-off staining/destaining procedure saves valuable time and reduces exposure to hazardous materials and solvent waste.

Blue-Clean Protein Stain exhibits sensitivity below 20ng of protein per band.

Packaged as a 1X ready-to-use solution, Blue-Clean Protein Stain only requires water for the prewashing and destaining procedures.

Procedure: Pre-wash gel with deionized water to denature gel for 15 minutes. Stain gel with Blue-Clean Protein Stain (completely submerge the gel) for 60 minutes. Then, destain with deionized water for 30 minutes or overnight.

CAS#:N/A
Appearance:Clear, Amber Solution
pH @ 25ºC:0.4-0.8
Abs. @ 465nm:0.7-1.0

Molecular Biology Specifications
Does not contain methanol or acetic acid.

IB01034 - Specification Sheet

Recommended Use
Hands off Simple Staining/Destaining Procedure
High Sensitivity, Below 20ng of Protein per Band

Safer Alternative, No More Methanol and Acetic Acid
Coomassie Blue Staining of proteins in SDS-PAGE gels is a daily procedure in many laboratories. It is popular among life science researchers, due to its good sensitivity and relative ease of use. Traditionally, Coomassie Blue staining requires a methanol and acetic acid solution to achieve staining and destaining. This process increases the risk for hazardous exposure and produces an unpleasant pungent odor. Blue-C Protein Stain is a convenient alternative to traditional Coomassie Blue staining procedures. Environmentally friendly, this ready-to-use stain does not contain methanol and acetic acid and does not require hazardous solvents for destaining. This simple “hands-off” staining/destaining procedure saves valuable time while reducing the handling of hazardous materials and solvent waste in your laboratory. Blue-C Protein staining exhibits sensitivity below 20ng of protein per band. Packaged as a 1x ready-to-use solution, Blue-C Protein only requires water for the prewashing and destaining steps.

Blue-Clean Protein Staining Procedure
15 min Pre-Wash with Deionized Water (for denaturing gels)
1 hour Staining with Blue-C Protein (completely submerge the gel)
30 min Destain in Deionized Water (may continue overnight)

Storage
Store at room temperature. Protected from moisture. Keep tightly sealed. Light Sensitive.

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