Blue Clean Protein Stain
- Item Description
- Physical Specifications
- Certificate of Analysis
- Technical Information
- Less hazardous alternative to Coomassie Blue Stain
- Exhibits sensitivity below 20ng of protein per band
- This product ships as a hazardous material
Blue-Clean Protein Stain is a more environmentally friendly alternative to traditional coomassie blue protein staining procedures in SDS-PAGE gels. Blue-Clean Protein Stain is a ready-to-use stain that does not contain methanol or acetic acid or require hazardous solvents for destaining. The simple hands-off staining/destaining procedure saves valuable time and reduces exposure to hazardous materials and solvent waste.
Blue-Clean Protein Stain exhibits sensitivity below 20ng of protein per band.
Packaged as a 1X ready-to-use solution, Blue-Clean Protein Stain only requires water for the prewashing and destaining procedures.
Procedure: Pre-wash gel with deionized water to denature gel for 15 minutes. Stain gel with Blue-Clean Protein Stain (completely submerge the gel) for 60 minutes. Then, destain with deionized water for 30 minutes or overnight.
Appearance:Clear, Amber Solution
pH @ 25ºC:0.4-0.8
Abs. @ 465nm:0.7-1.0
Molecular Biology Specifications
Does not contain methanol or acetic acid.
Hands off Simple Staining/Destaining Procedure
High Sensitivity, Below 20ng of Protein per Band
Safer Alternative, No More Methanol and Acetic Acid
Coomassie Blue Staining of proteins in SDS-PAGE gels is a daily procedure in many laboratories. It is popular among life science researchers, due to its good sensitivity and relative ease of use. Traditionally, Coomassie Blue staining requires a methanol and acetic acid solution to achieve staining and destaining. This process increases the risk for hazardous exposure and produces an unpleasant pungent odor. Blue-C Protein Stain is a convenient alternative to traditional Coomassie Blue staining procedures. Environmentally friendly, this ready-to-use stain does not contain methanol and acetic acid and does not require hazardous solvents for destaining. This simple “hands-off” staining/destaining procedure saves valuable time while reducing the handling of hazardous materials and solvent waste in your laboratory. Blue-C Protein staining exhibits sensitivity below 20ng of protein per band. Packaged as a 1x ready-to-use solution, Blue-C Protein only requires water for the prewashing and destaining steps.
Blue-Clean Protein Staining Procedure
15 min Pre-Wash with Deionized Water (for denaturing gels)
1 hour Staining with Blue-C Protein (completely submerge the gel)
30 min Destain in Deionized Water (may continue overnight)
Store at room temperature. Protected from moisture. Keep tightly sealed. Light Sensitive.