20% Acrylamide-UREA Solution

20% Acrylamide-Urea Solution in TBE Buffer – Ready-to-Pour for PAGE Gel Electrophoresis

Our 20% Acrylamide-Urea Solution in TBE buffer is a high-performance, ready-to-pour solution designed for casting denaturing polyacrylamide gels. Formulated with a 19:1 acrylamide to bis-acrylamide ratio, this solution ensures consistent gel polymerization and is ideal for a wide range of PAGE gel electrophoresis applications in molecular biology.

Researchers rely on this formulation for its ease of use, time-saving convenience, and reproducible results in high-resolution nucleic acid separations.

Applications in DNA, RNA, and tRNA Electrophoresis

This acrylamide-urea solution is widely used in DNA and RNA analysis, including separation of restriction enzyme digests, PCR products, and fragments used in Southern blotting or primer extension assays. It also supports nucleic acid sequence analysis, offering the resolution necessary to distinguish short single-stranded DNA or RNA fragments that differ by as little as one nucleotide.

Additionally, the solution is well-suited for electrophoretic separation of tRNA isoforms, enabling differentiation based on molecular charge, conformation, or size. Its high clarity and denaturing properties make it ideal for sensitive applications requiring accuracy and consistency in nucleic acid electrophoresis.