15% Acrylamide-UREA Solution

15% Acrylamide-Urea Solution in TBE Buffer – Ready-to-Pour for PAGE Gels

Our 15% Acrylamide-Urea Solution in TBE buffer is a pre-mixed, ready-to-pour formulation made with a 19:1 acrylamide to bis-acrylamide ratio. This high-purity solution is designed for polyacrylamide gel electrophoresis (PAGE) and is ideal for critical nucleic acid applications requiring high-resolution separation. It simplifies the gel casting process for researchers working with oligonucleotides, RNA analysis, and transcription studies.

This product is specifically optimized for PAGE-based applications including oligonucleotide purity analysis, RNase protection assays, in vivo transcription experiments, and northern blotting. With minimal prep time, users only need to add ammonium persulfate and TEMED to initiate polymerization. Detailed instructions are included to ensure optimal gel casting results. This solution supports reproducibility, clarity, and consistency in lab workflows and is classified as hazardous, requiring proper handling.

Reliable Performance for RNA and DNA Gel Electrophoresis

Formulated for precision, the 15% Acrylamide-Urea Solution provides consistent gel matrix quality, ensuring uniform migration and resolution of small RNA and DNA fragments. Its formulation supports stable urea concentrations, maintaining denaturing conditions critical for accurate separation. Whether analyzing oligonucleotide purity or performing RNase protection assays, this solution delivers dependable electrophoretic performance.

To make a gel, combine 1 mL of the 15% Acrylamide-Urea Solution with 5 µL of 10% ammonium persulfate solution. Add 1.25 µL of TEMED last, as it initiates polymerization. The mixture should be poured immediately to ensure a smooth, homogenous gel. Polymerization typically completes within 15 to 60 minutes, depending on ambient conditions. It's recommended to prepare a small extra volume of the gel mix and leave it in the mixing tube. This will serve as a visual cue to determine when the gel has fully polymerized and is ready for loading.

Convenient, Pre-Mixed Gel Solution for Efficient Lab Use

This ready-to-pour acrylamide solution eliminates the need for labor-intensive preparation and reduces variability in gel casting. Researchers benefit from standardized results, faster prep times, and reliable performance across experiments. Suitable for academic, biotech, and clinical research settings, this formulation streamlines the PAGE process while supporting high-resolution results for nucleic acid analysis.