TAE (Tris / Acetate / EDTA) buffer is commonly used in low voltage DNA electrophoresis applications. It’s relatively low buffering capacity will become exhausted in extended or current electrophoresis runs making buffer recirculation necessary in such runs.
- Item Description
- Physical Specifications
- Certificate of Analysis
- SDS
Additional Item Description:
Potential advantages of TAE over TBE buffer include superior resolution of supercoiled DNA and slightly faster migration of double stranded DNA fragments.
10 X TAE Buffer Concentrate will produce 10 L of 1 X solution, but smaller quantities can be produced. Electrophoresis buffer contains Tris, Acetate, and EDTA.
Physical Attributes:
CAS#:N/A
Solution Components:
- 0.4 M Tris-Acetate
- 0.01 M EDTA
pH (1 x solution @ 25°C):8.3 ± 0.1
Molecular Biology Specifications:
Gel Analysis assay: Pass