Tetracycline Hydrochloride
Model Number IB02200
For research use only. Not for human, veterinary, or therapeutic use. Sold as a laboratory selection reagent. CAS 64-75-5. Used in molecular biology to select E. coli carrying tetracycline-resistance plasmids and as an inducer in Tet-On/Tet-Off expression systems.
Tetracycline Hydrochloride inhibits bacterial protein synthesis by preventing aminoacyl-tRNA from binding to the ribosome. It is used to select bacteria carrying tetracycline-resistance genes. A typical stock solution is 5 mg/ml in water. If dissolved in ethanol, the solution does not need to be sterilized. Magnesium ions block tetracycline, so use media without magnesium salts, such as LB medium, for selection. Potency minimum 900 µg/mg.
- Item Description
- Physical Specifications
- Certificate of Analysis
- SDS
CAS#: 64-75-5
- For use in cell culture media to inhibit gram-positive and gram-negative bacteria
- This product cannot be shipped to a private residence.
Tetracycline Hydrochloride is an antibiotic that inhibits protein synthesis (elongation) by preventing the binding of amino-tRNA to ribosome. Tetracycline is for use in cell culture applications.
The tetracycline powder is used to make a stock solution at 5mg/ml in water. The working concentration for stringent plasmids is 10µg/ml and 50µg/ml for relaxed plasmids.
If dissolved in ethanol, the antibiotic solution need not be sterilized.
Magnesium ions are antagonists of tetracycline. Use media without magnesium salts (e.g. LB medium) for the selection of bacteria resistant to tetracycline.
CAS#:64-75-5
Molecular Formula:C22H24N2O8HCl
Molecular Weight:480.90
Potency:Min. 900 μg/mg
pH (1%, water):2.3 ±0.5
Moisture:Max. 2.0%
4-ephianhydrotetracycline:Max. 2.0%
Molecular Biology Specifications
Antibiotic Inhibition/Sensitivity Assay:Pass
RELATED PRODUCTS
Terrific Broth
$66.03
Yeast Extract
$77.19
LB Miller Broth
$70.19
Bacteriological Agar
$155.30
Tryptone
$94.63
Dri-Gauze Filter Cap Linings (Flask) - 5 Pack
$34.38
Full-Baffle TUNAIR™ Polypropylene Erlenmeyer Flask Kit, 2.5 Liters
$186.96
IPTG (Isopropyl β-D-thiogalatopyranoside)
$35.9325% off your order
Enter your email address to get 25% off your order and free shipping today
