• Contains casein digest peptone, sodium chloride, and yeast extract
• For maintaining and propagating E.coli culture

IBI’s LB Lennox Broth is used in molecular genetic studies. It is a nutritionally rich media for the growth and maintenance of pure cultures of recombinant strain of E. coli. The inclusion of casein peptone and yeast extract supplies essential growth factors to the E. coli bacterial culture; such as nitrogen, carbon, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes.

To prepare, mix 20 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store at room temperature, tightly sealed, and protect from moisture and light.

Physical Specifications
CAS#: N/A
pH (@ 25°C): 7.0 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications
Escherichia coli ATCC 23724: Growth
Escherichia coli ATCC 33694: Growth
Escherichia coli ATCC 33849: Growth
Escherichia coli ATCC 39403: Growth
Escherichia coli ATCC 47014: Growth
Escherichia coli ATCC 53868: Growth

LB Lennox Broth Specification Sheet

LB Lennox Broth CofA Lot# 18C1014

LB Lennox Broth CofA Lot# 18B3013

LB Lennox Broth CofA Lot# 17L1004

LB Lennox Broth CofA Lot# 17K5020

LB Lennox Broth CofA Lot# 17H4013

LB Lennox Broth CofA Lot# 17G4014

LB Lennox Broth CofA Lot# 17C5001

LB Lennox Broth CofA Lot# 16L3004

LB Lennox Broth CofA - Lot# 16I5015

LB Lennox Broth CofA - Lot# 160204032002

LB Lennox Broth CofA - Lot# 160219042002

LB Lennox Broth CofA Lot# J15-44

LB Lennox Broth CofA Lot# F15-58

LB Lennox Broth CofA Lot# C15-46

LB Lennox Broth CofA Lot# K14-02

LB Lennox Broth CofA Lot# H14-46

LB Lennox Broth CofA Lot# G14-37

LB Lennox Broth CofA Lot# E14-08

LB Lennox Broth CofA Lot# 18F5015

LB Lennox Broth CofA Lot# 18G4015

LB Lennox Broth CofA Lot#18H5002

LB Lennox Broth CofA Lot#18J2005

LB Lennox Broth CofA- Lot#19A3031

LB Lennox Broth CofA - Lot_19C4004

LB Lennox Broth CofA- Lot_19D3012

LB Lennox Broth CofA- Lot_19D3002

LB Lennox Broth CofA- Lot_19I5011

LB Lennox Broth CofA - Lot#20A3005

LB Lennox Broth CofA_-_Lot#20C5001

LB Lennox Broth CofA_-_Lot#20K3003

LB Lennox Broth CofA -_Lot#20L4022

LB Lennox Broth CofA_-_Lot#21D5004

LB Lennox Broth CofA - Lot#21E2015

LB Lennox Broth CofA - Lot#21E2025

LB Lennox Broth CofA - Lot#22E4035

LB Lennox Broth CofA - Lot#22E4003

LB Lennox Broth CofA - Lot#22K3013

LB Lennox Broth CofA - Lot#23C1023

LB Lennox Broth CofA - Lot#23M4002

LB Lennox Broth CofA - Lot#24C3014

LB Lennox Broth - SDS

Formula per Liter
● Casein Digest Peptone 10.0 gm
● Sodium Chloride 5.0 gm
● Yeast Extract 5.0 gm

Preparation
● Mix 20 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use
IBI’s LB Lennox Broth is used in molecular genetic studies. It is a nutritionally rich media for the growth and maintenance of pure cultures of recombinant strains of E.coli. The inclusion of casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins. Sodium chloride also provides essential electrolytes.

Storage
Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.