Standard Agarose

$48.47

Standard Agarose Product Description:

IBI Standard Agarose (Molecular Biology Grade Agarose) products are molecular biology certified for use in standard electrophoresis procedures including nucleic acid, analytical, and preparative electrophoresis and blotting. IBI Standard Agarose is highly refined and purified with strict testing parameters to ensure lot-to-lot consistency. IBI Standard Agarose is a Low EEO agarose with high electrophoresis mobility characteristics.

IBI Standard Agarose is a neutral polysaccharide extracted from the cell walls of certain Rhodophyceae which are also known as agarophyte seaweeds. The structure of the polysaccharide is a galactan, formed by linking agarobioses 1-3, 1-4. This chemical structure gives agarose the capacity to form gels that are very strong even at low concentrations. These gels have a macroreticular structure with a very open mesh which can be adjusted simply by varying the concentration of agarose.

Additional Features:

CAS#: 9012-36-6
Extraordinary mechanical resistance
Low absorption of staining reagents
Excellent transparency for high visibility
Greater thermal stability due to high hysteresis
Lot-to-lot consistency
DNase- and RNase-Free
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What is Agarose?

Agarose, a vital polysaccharide is carefully purified and isolated from agar or seaweed. It consists of interchanging units of D-galactose and 3,6-anhydro-alpha-L-galactopyranose residues interconnected by alpha-(1- >3)- and beta-(1- >4)-linkages in its structure.

What is Agarose used for?

Agarose is commonly used as a gelling agent, and can be applied towards electrophoresis, which is a widely used lab technique that requires biological molecule extraction.

What are the Key Properties of Agarose?

Strength of Gel - The power required to break the gel.

Content of Sulfate - To indicate purity, Sulfate is utilized because it is the notable ionic group present.

EEO (Electroendosmosis) - Electroendosmosis occurs when a liquid is transferred through a gel. Agarose gel anionic groups are affixed and the counterions that are dissociable travel to the cathode, inducing EEO. Since biopolymers usually gravitate towards an anode while electrophoresis is occurring, the EEO effect can disrupt separation by way of convection internally.

Gel Point - The gelation temperature of agarose in water is when it solidifies upon cooling. There’s hysteresis in agarose solutions during the transition from liquid to gel, meaning the gelation temperature differs from the melting temperature.

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Physical Specifications include:

CAS#: 9012-36-6
Moisture: ≤ 7%
Ash: ≤ 0.4%
EEO: 0.05-0.13
Sulfate: ≤ 0.10%
Clarity @ 1.5% (NTU): ≤ 3
Gel Strength @ 1.0% (g/cm2): ≥ 1200
Gel Strength @ 1.5% (g/cm2): ≥ 2500
Gelling Temperature @ 1.5%: 36°C ± 1.5°C
Melting Temperature @ 1.5%: 88°C ± 1.5°C

Molecular Biology Specifications
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Gel analysis assay: Pass
DNA Resolution: ≥ 1000bp (Finely Resolved)
DNA Extraction assay: Pass
Gel Background: Very Low