Ethylenediaminetetraacetic acid (EDTA) Solution

Model Number IB70184

  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • Technical Information
  • SDS

CAS# 6381-92-6

  • For use in biological and electrophoresis buffer systems
  • Available in 100ml and 400ml sizes
  • For Research Use Only. Cannot be shipped to a personal residence.

IBI 0.5MEDTA solution (pH 8.0)is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions.

Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.

IBI offers EDTA solutionin 100ml and 400ml sizes.

For Research Use Only - Not For Human Consumption.

Molecular Formula:C10H14N2O8Na2x 2H2O
Purity (EDTA component; dry basis):>99.0%
Heavy Metals:<0.005%
A280-600 (0.5 M, water):<0.10%
A 0.5 M aqueous solution of a Molecular Biology Certified Grade EDTA pH adjusted to 8.0

Molecular Biology Specifications
DNase Assay:None Detected
RNase Assay:None Detected
Protease Assay:None Detected

IB70184, IB70185, IB70187 - Specification Sheet


Mol. Wt.: 412.3   EDTA, 0.5 M, pH 8.0, CAS 150-38-9
Ethylenediaminetetraacetic Acid Trisodium Salt  
C10H13N2Na3O8 · 3H2O

EDTA (ethylenediamenetetraacetic acid)


IBI EDTA solution is prepared as follows:   EDTA at 0.5 M (pH 8.0): Add 186.1 g of disodium EDTA•2H2O to 800 mL of H2O. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (~20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving. The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to ~8.0 by the addition of NaOH.

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