• Contains casein digest peptone, yeast extract, Potassium phosphate – monobasic, Potassium phosphate – dibasic
• For use in cultivating recombinant strains of E.coli

IBI’s Terrific Broth is used with Glycerol in cultivating recombinant strains of E. coli. Terrific broth powderis a highly enriched medium for improving yield in plasmid bearing E. coli. Recombinant strains have an extended growth phase in the medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.

CAS#: N/A
pH (@ 25°C): 7.2 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications
Escherichia coli ATC 23724: Growth
Escherichia coli ATC 33694: Growth
Escherichia coli ATC 33849: Growth
Escherichia coli ATC 39403: Growth
Escherichia coli ATC 47014: Growth
Escherichia coli ATC 53868: Growth

Terrific Broth Specification Sheet

Terrific Broth CofA - Lot# 17L1034

Terrific Broth CofA - Lot# 17K5030

Terrific Broth CofA - Lot# 17K1004

Terrific Broth CofA - Lot# 17D2004

Terrific Broth CofA - Lot# 16I5025

Terrific Broth CofA - Lot# 16F5002

Terrific Broth CofA - Lot# 160129012001

Terrific Broth CofA - Lot# I15-20

Terrific Broth CofA - Lot# I15-18

Terrific Broth CofA - Lot# D15-99

Terrific Broth CofA - Lot# A14-10

Terrific Broth CofA - Lot# E14-13

Terrific Broth CofA - Lot# 18F5025

Terrific Broth CofA- Lot#18L4021

Terrific Broth CofA- Lot#18L3001

Terrific Broth CofA- Lot#19B3024

Terrific Broth CofA- Lot_19G5021

Terrific Broth - SDS

Formula per Liter
● Casein Digest Peptone 12.0 gm
● Yeast Extract 24.0 gm
● Potassium Phosphate, Monobasic 2.3 gm
● Potassium Phosphate, Dibasic 12.5 gm

Preparation
● Mix 50.8 gm of the medium in 1 L of purified water until evenly dispersed.
● Add 4 ml of Glycerol to the mixture.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use
IBI’s Terrific Broth is used with Glycerol in cultivating recombinant strains of E.coli. Terrific broth is a highly enriched medium for improving yield in plasmid bearing E.coli. Recombinant strains have an extended growth phase in the medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.

Storage
Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.