5 Things You Should Know When Choosing a DNA Test Kit

Posted by Katie Swoboda on

When it comes to DNA purification, there are many DNA test kit options on the market. However, not all DNA kits are created equal. Furthermore, what works well for one setting may be completely wrong for another laboratory workflow. Consider the following factors when selecting a DNA extraction kit:

 

  • Quality of reagents. The reagents determine the quality of the results you will get from a DNA extraction kit. Read the technical specifications carefully to determine how cells are lysed and protein degraded. Use of a high-quality wash buffer is especially important to ensure the final purified genomic DNA is free of contaminants.

 

  • Suitability for genomic DNA. Not all test kits are designed to process genomic DNA. When choosing a kit, check to make sure it is optimized for use with genomic DNA. Should you require RNA purification, viral RNA or DNA extraction, or plasmid DNA, other products will be more suitable.

 

  • Always consider the target yield for a given kits. Preps that process large amounts of blood may yield up to 50 µg of purified DNA. If you require a larger throughput or have smaller samples, yields of 5 to 15 µg may be acceptable. Consider the downstream experiments you intend to perform to choose the appropriate yield.

 

  • We offer mini genomic DNA sample kits for low-throughput operations as well as kits with 300 or more preps, 96-well plates, and other high-throughput options. This allows you to remain flexible while ensuring scalability of your DNA extraction pipeline.

 

  • Operation time. When the success of your experiments depends on purifying DNA, you want to achieve the best possible results within a feasible timeline. For example, our 96-well Blood Genomic DNA kit offers a 45-minute completion time, ensuring that you can maintain an efficient workflow.

Share this post



← Older Post