Semi-Dry Blotter

• Ideal for efficient and economic transfer of protein, RNA, or DNA from polyacrylamide or agarose gels to a solid membrane support, which is subsequently visualized by staining methods
• Working Surface Dimensions: 1
  • IB44000 = 16cm x 16cm 
  • IB45000 = 25cmx 30cm
• Recommended Maximum Capacity:
  • IB44000 = 1 - 6 Trans-Units
  • IB45000 = 1 - 3 Trans-Units

The IB44000 and IB45000 semi dry blotters have plates that are graphite coated to reduce heat, minimize buffer usage and produce clear transfer bands that are not distorted.

A transfer unit stack is formed by the gel and membrane being in contact and sandwiched between filter papers soaked in transfer buffer. An electric current is applied perpendicular to the gel and buffer saturated filter papers. The bands from the charged molecules of the gels migrate and bind to the membrane to complete the transfer. The time required to complete the transfer is dependent on molecule size and the number of transfer units being transferred.

Several stacks of transfer units can be run simultaneously.

• The IB44000 semi-dry blotter can accommodate six trans-units at the same time
• The IB45000 semi-dry blotter can accommodate three trans-units at the same time

IB44000

Working surface dimensions: 16.0cm(W) x 16cm(L)
Weight: 3.75 lbs.
Cathode electrode wire: 36 inches
Anode electrode wire: 36 inches
Recommended capacity: 1 - 6 trans-units.
Electrode plate material: graphite

IB45000

Working surface dimensions: 24cm(W) x 30cm(L)
Weight: 9.5 lbs.
Cathode electrode wire: 36 inches
Anode electrode wire: 36 inches
Recommended capacity: 1 – 3 trans-units.
Electrode plate material: graphite

IB44000/IB45000 - Semi-Dry Blotter Operation Manual

Current Density --- Trans Units --- Time Limit
0.8ma/cm2 --------------- 1 to 6 --------------- 1 to 2 hours
2.5ma/cm2 --------------- 1 to 6 --------------- 30 – 45 minutes
1.0ma/cm2 --------------- 1 to 6 --------------- 10 – 30 minutes