Model Number IB07200


Tween 20 is a non-ionic detergent widely used in biochemical applications. It can be used as an emulsifying agent to prepare stable oil-in-water emulsions. TWEEN 20 can be used to remove peripheral protein from membranes. TWEEN 20 is commonly used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%.

  • Additional Item Description
  • Physical Specifications
  • Technical Information
  • Certificate of Analysis
  • SDS

Product Details:

  • CAS#  9005-64-5
  • 500ml Bottle of Tween 20 OR 1 liter bottle of TWEEN 20
  • A non-ionic detergent
  • DNase, RNase and Protease free

Physical Specifications:

Appearance: Yellowish liquid

Hydroxyl Value: 96-108

Saponification Value: 40-50

Moisture: < 3.0%

Dioxane: < 10ppm

Acid Value: < 2.0

Gardner Color: < 5.0

BSE/TSE Free: Pass

Country of Origin: U.S.A.

View/Download Specifications Sheet

TWEEN 20 Technical Info:

TWEEN 20 is polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a non-ionic detergent used commonly in biochemical applications. 

TWEEN 20 can be stored at room temp in a cool dry area and the bottle should be sealed tightly when not in use. 

IBI TWEEN 20 can be used as washing agent immunoassays such as Western Blots and ELISAs. It helps prevent non-specific antibody binding. In these applications it is typically dissolved in Tris-Buffered saline (TBS) or Phosphate Buffered saline (PBS) at dilutions of 0.05% in 1X or 0.5% in 10X solution. These buffers are used for washes between each immune-reaction, to remove unbound immunologicals, and eventually for incubating solutions of immuno-reagents such as labeled antibodies in order to reduce nonspecific background. 

For premixed PBS with TWEEN 20 see IBI model numbers IB70167 and IB70168

TWEEN 20 is commonly used as a component of:

  • Blocking buffer in immunohistochemistry
  • Washing buffer and blocking buffer in Western Blotting
  • Washing buffer in ELISA (Enzyme Linked Immunosorbent Assay)
  • Reaction mixture in PCR  (Polymerase Chain Reaction)

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