Chloramphenicol Antibiotic

Model Number IB02080

  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS
  • Technical Information

CAS#: 56-75-7

  • For use in cell culture media as a broad spectrum antibiotic

Chloramphenicol antibiotic is a broad spectrum antibiotic, active against a wide variety of gram positive and gram negative bacteria, including most anaerobic organisms. This antibiotic blocks bacterial protein synthesis by inhibiting the peptidyl transferase activity of the 50S ribosomal subunit (elongation inhibition).

The antibiotic powder is used to make a stock solution at 34mg/ml in ethanol. The working concentration for stringent plasmids is 25µg/ml and 170µg/ml for relaxed plasmids.

If dissolved in ethanol, the chloramphenicol solution need not be sterilized.

CAS#: 56-75-7
Molecular Weight: 323.13
Molecular Formula: C11H12Cl2N2O5
Melting Point: 149-153°C
pH (2.5%, water): 6.0 ± 1.5
Purity: 97.0 – 103.0%

Molecular Biology Specifications
Antibiotic Inhibition/Sensitivity Assay: Pass
Identification: Pass
Crystallinity: Pass
Chromatographic Purity: Pass

IB02080 - Specification Sheet

Can you make a stock solution of Chloramphenicol in water?

Chloramphenicol antibiotic dissolves best in alcohol or benzene. It does not dissolve well in water. A stock solution in alcohol should be made at 10 mg/ml and then diluted with water to 1 mg/ml. Stock solutions made up of strictly water (0.25% = 7.7 mM) will degrade in sunlight, UV light or tungsten light at or near room temp over a period of 96 hours. It does NOT degrade when dissolved in alcohol or benzene.

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