Mini Select-D w/G25 & TE

Model Number IB06000

  • Item Description
  • Specifications
  • Protocol
  • Retention of unincorporated NTPs: up to 90%
  • Operation time: up to 10 minutes
  • DNase- and RNase-free

The Mini Select-D TE, G-25 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>12mer), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5’ end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Mini Select-D TE, G-25 spin columns are RNase-free and can be used for rapid purification of oligoribonucleotides and RNA from unincorporated ribonucleotides (rNTPs) and in other related applications. After a brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.

Each DNase- and RNase-free column is prepackaged with Sephadex G-25 in sterile TE buffer. Two nuclease-free collection tubes (autoclavable) are supplied for each column.

Specification Sheet

Sample Size:Up to 20ul
Format:Gel filtration in spin column
Carrier Buffer:TE
G25 Sephadex inside spin column containing frit filter. Column capped on both ends.

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