Viral Nucleic Acid Extraction Sample Kit – 4 Preps
- Sample Size: 200µl sample (plasma, serum, body fluid, or supernatant of viral infected cell cultures)
- Operation Time: 40min or less
- Elution Volume: 50µl
- Free sample available upon request
The Viral Nucleic Acid Extraction Kit was designed specifically for purification of viral DNA/RNA from cell-free samples such as: serum, plasma, body fluids, and the supernatant of viral infected cell cultures. DNA/RNA viruses are lysed quickly and efficiently using the lysis buffer, which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates an optimum condition for nucleic acid binding to the glass fiber of the column. Contaminants such as salts, metabolites, and soluble macromolecular cellular components are removed in the wash process. The nucleic acids are eluted in RNase-Free water and are then ready for use in subsequent reactions including Real-Time PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of the individual PCR or RT-PCR assays.
The quality of the Viral Nucleic Acid Extraction Kit is tested on a lot-to-lot basis by isolating viral DNA/RNA from a 200µl plasma sample.
I can’t see any RNA, but I do see DNA. Is this a problem with concentrations? Remember to use RNAse free centrifuge tubes, tips, and other tools, and always wear disposable gloves during all of the extraction processes. The low concentration samples can lead to not being able to see RNA on the gel. To check the result, you can perform the “Reverse Transcription PCR.”