TUNAIR™ Shake Flask Sample Kit
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TUNAIR™ Shake Flask Sample Kit

  • $202.40 Model Number SS-7001



  • Complete with (1) polypropylene 300ml No-Baffle TUNAIR Shake Flask, (1) polypropylene 300ml Half-Baffle TUNAIR Shake Flask, (1) polypropylene 300ml Full-Baffle TUNAIR Shake Flask, (3) 300ml two piece caps, (1) 300ml silicone cap lining, (2) 300ml dri-gauze cap linings, (1) polypropylene 2.5L Full-Baffle TUNAIR Shake Flask, (1) 2.5L two piece cap, (1) 2.5L silicone cap lining .

The TUNAIR™ Sample Kit provides a great opportunity to try all of the flasks, filters, and caps with your cell culture!

The TUNAIR™ Shake Flask Systems are a unique and patented flask and closure system, designed for microbiology and biotechnology applications. This system provides optimum growth conditions for aerobic microorganisms, mammalian cells, and plant cells. They also provide better culture growth and productivity than standard Erlenmeyer flasks. The TUNAIR™’s high oxygen absorption rate is due to the unique baffling and turbo-vane closure design. The TUNAIR™ systems are designed to increase the availability of dissolved oxygen as well as improve cell yields.

The Full-Baffle Shake Flask has six baffles that produce a propeller motion. The Half-Baffle Shake Flask has two baffles that produce a vortex motion. The No-Baffle Shake Flask produces a normal throw similar to an Erlenmeyer flask.

The working volume of the 300ml flask is 100ml, and the working volume of the 2.5L flask is 1L.

The silicone filters are 0.22 micron and will last indefinitely unless punctured. The silicone filters do not need to be washed, but can be rinsed with water if visibly soiled. It is recommended to always leave the filter in the cap and autoclave. The Dri-Gauze filter is 0.22 micron and constructed of nitrocellulose. Each filter can be used 10 – 12 times. The Dri-Gauze filters can be autoclaved in the cap.

The two piece cap assemblies are constructed of polypropylene and are resistant to most solvents. All flasks and caps are fully autoclavable prior to reuse and filter linings can also be autoclaved or simply replaced. To replace the filter lining in the cap assembly, simply pinch the flanges of the inner-closure shell until they snap loose. Then, pull apart and remove used lining. Replace the lining by sandwiching it between two parts of the cap and snap the cap back together. When reassembling the cap, ensure the flanges from the inner piece snap into the mated grooves in the outer piece. This will ensure the cap assembly stays together during use.

All TUNAIR™ caps can be cleaned by soaking in water with a light detergent solution to loosen dirt and contaminants, then air dry. 

Flask Dimensions:
Flask Size: 300ml
Working Volume: 100ml
Base Diameter: 3.25” [8.25cm]
Neck Diameter: 1.75” [4.45cm]
Height: 6.00” [15.24cm]
Weight: 0.01 lbs. [0.004Kg]

Flask Dimensions:
Flask Size: 2.5L
Working Volume: 1L
Base Diameter: 6.50” [16.51cm]
Neck Diameter: 3.00” [7.62cm]
Height: 10.75” [27.31cm]
Weight: 0.02 lbs. [0.008Kg]

Mixing:
No-Baffle (0 Baffles): Normal Throws
Half-Baffle (2 Baffles): Vortex Motion
Full-Baffle (6 Baffles): Propeller Motion

Shaker Speed:
1” Throws: 300-400rpm or possibly higher
2” Throws: 150-200rpm or possibly higher

Material:
All TUNAIR flasks and caps are constructed of chemical resistant polypropylene. All flasks and caps are fully autoclavable.

Cleaning:
All TUNAIR flasks and caps can be cleaned by soaking in water with a light detergent (i.e. Joy) solution to loosen dirt and contaminants; air dry. All TUNAIR flasks and caps can be autoclaved, if required.

Cell Growth Evaluation of Commonly Used Shake Flasks

TUNAIR™ flasks were compared to conventional flasks using four different types of microorganisms; Escherichia coli, Saccharomyces cerevisiae, Penicillium avellaneum, and Streptomyces chartreusis. The aeration capacities of the shake flasks were determined by the sulfate oxidation method, and the values shown below are presented as oxygen absorption rate (OAR) in mM oxygen/L/Min. The growth rates of E.coli and S.cerevisiae were expressed as optical densities (OD) at 555mM. For S.chartreusis and P.avellaneum growth rates were evaluated by percent sedimentation. For E.coli and S.cerevisiae, the growth rates were determined after an 18-hour incubation period; for S.charteusis, a 24-hour incubation period; and for P.avellaneum, a 72-hour incubation period. Growth and OAR evaluations were carried out with 3-9 replicates and statistically analyzed using Turkey’s w-procedure. See results below.

Growth Evaluation of Four (4) Microbial Types in TUNAIR™ Flasks vs. Other Currently Used Shake Flasks
OAR Value OD @ 555mM % Sedimentation
Flask mM O2/L/Min. E.coli S.cerevisiae S.chartreusis P.aveilaneum
TUNAIR™ Full-Baffle 4.25 7.09 5.63 19.7 3.3M
TUNAIR™ Half-Baffle 1.22 5.36 5.57 27.73 30.50P
Triple Indented Flasks 2.47 5.97 5.31 19.20 9.50MP
Unbaffled Erlenmeyer 0.52 5.97 5.19 17.37 25.10P

*Growth Morphology: M, mycelial; P, pellet; MP, mixed mycelial. The mycelial growths mostly adhered to the walls of the flask, which accounted for the low overall sedimentation value.

 

Growth Comparison of Saccharomyces Cerevisiae in TUNAIR™ Shake Flasks and Brand C Shake Flasks

The experiment was done on a New Brunswick INNOVA 44 shaker incubator.
It was conducted at different speeds – 200rpm & 300rpm.
Strain: Saccharomyces Cerevisiae
Medium: YPD broth (Yeast Extract Peptone Dextrose)
Flasks: IBI TUNAIR™ 300ml Flask and Brand C 250ml Growth Flasks
Cell Analyzer: Vi-Cell XR

 

S.Cerevisiae Growth Chart

In this experiment, standard volume--60 & 50ml YPD--was used for TUNAIR™ and Brand C flasks, which is 20% capacity of the flasks. The flasks were incubated at 30°C at speeds of 200rpm and 300rpm for 28 hours. After taking viable cell counts, it was found that under low speed (200rpm) both TUNAIR™ and Brand C flasks contained yeast cultures of higher cell densities when compared to the higher speed (300rpm) cell culture flasks. It was also noted that the TUNAIR™ flask had higher cell density at 200rpm and 300rpm when compared to the Brand C flask. Although these data indicate TUNAIR™ flasks support higher density cell growth, we expected that higher speed (rpm) should produce higher cell density due to the higher dissolved oxygen concentration and better dispersion of cells. This unexpected data might be due to frozen cell culture stock, which might take a longer time to adapt to the environment.

More experiments are required and are being carried out at this time. The focus of these experiments will involve:
• Varying shaker speeds and varying volumes of medium in the TUNAIR™ and Brand C flasks
• Comparative studies between TUNAIR™ 2.5-L growth flasks and Brand C 2-L flasks
• Varying shaker speeds and varying volumes of medium in the larger TUNAIR (2.5L) and larger Brand C (2L) flasks

Reference Papers:

1. Method to Increase the Yield of Eukaryotic Membrane Protein Expression in Saccharomyces Cerevisiae

2. Optimisation of Recombinant Production of Active Human Cardiac SERCA2a ATPase