Tryptose Phosphate Broth

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Tryptose Phosphate Broth

Model Number IB49151

$59.14

  • Item Description
  • Physical Specifications
  • Certificate of Analysis
  • SDS
  • Technical Information
  • Contains casein digest peptone, yeast extract, Potassium phosphate – monobasic, Potassium phosphate – dibasic
  • For use in cultivation of fastidious microorganisms including streptococci and meningococci

IBI’s Tryptose Phosphate Broth is a medium recommended for the cultivation of fastidious microorganisms. Tryptose phosphate broth is a buffered broth which can support the growth of fastidious streptococci and meningococci. The addition of 0.1% agar concentration can aid in the recovery of obligate anaerobes. Tryptose peptone and dextrose are a source of carbon, organic nitrogen, and energy. Sodium chloride contributes to osmotic equilibrium and disodium phosphate is a buffering agent.

To prepare, mix 50.8 g of the medium in 1 L of purified water until evenly dispersed. Add 4 ml of Glycerol to the mixture and evenly disperse. Heat with repeated stirring to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store product at room temperature, keep tightly sealed, and protect from moisture and light.

CAS#: N/A
pH (@ 25°C): 7.3 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications
Neisseria Meningitidis ATCC 13090: Growth
Stepylococcue Epidermidis ATCC 12228: Growth
Streptococcus Pneumonia ATCC 6305: Growth
Streptococcus Pyogenes ATCC 19615: Growth

Tryptose Phosphate Broth Specification Sheet

Formula per Liter
● Tryptose 20.0 gm
● Dextrose 2.0 gm
● Disodium Phosphate 2.5 gm
● Sodium Chloride 5.0 gm

Preparation

● Mix 29.5 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use
IBI’s Tryptose Phosphate Broth is a medium recommended for the cultivation of fastidious microorganisms. Tryptose phosphate broth is a buffered broth which can support the growth fastidious streptococci and meningococci. The addition of 0.1 agar concentration can aid in the recovery of obligate anaerobes. Tryptose peptone and dextrose are a source of carbon, organic nitrogen, and energy. Sodium chloride contributes to osmotic equilibrium, and disodium phosphate is a buffering agent.

Storage
Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.

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