• Used for isolation of different strains of fastidious microorganisms

IBI’s Trypto Soy Agar is a general growth medium prepared with or without blood or enrichments. It is used for the isolation of different strains of fastidious microorganisms.

Store at room temperature (less than 30⁰C), tightly sealed, and protect from moisture and light.

Physical Specifications
Cas#: N/A
pH (@ 25°C): 7.3 ± 0.2
Note: Grams per/liter may be adjusted to achieve desired performance.

Molecular Biology Specifications
Eschericha coli ATCC 25922: Growth
Straphylococcus aureus ATCC 25923: Growth
Streptococcus pneumonia ATCC 6305: Growth
Streptococcus pyogenes ATCC 19615: Growth

Trypto Soy Agar Specification Sheet

Trypto Soy Agar CofA Lot#18A5033

Trypto Soy Agar CofA Lot# A15-01

Trypto Soy Agar CofA Lot# H14-33

Trypto Soy Agar CofA Lot# D14-07

Trypto Soy Agar CofA Lot#18H5012

Trypto Soy Agar CofA Lot#19A3001

Trypto Soy Agar - SDS

Formula per Liter
● Casein Digest Peptone 15.0 gm
● Agar 15.0 gm
● Papaic Digest of Soybean Meal 5.0 gm
● Sodium Chloride 5.0 gm

Preparation
Mix 40.0 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring
and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. To prepare
5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium
cooled to 45-50°C. For chocolate agar; prepare single strength agar medium and cool to 45-50°C after sterilization.
Add 5 to 10% sterile defibrinated blood and heat to 80°C for 10 minutes.

Recommended Use
Trypto Soy Agar is a general growth medium prepared with or without blood or enrichments. It is used for the
isolation of different strains of fastidious microorganisms.

Storage
Store at room temperature (less than 30°C). Keep tightly sealed. Protect from moisture and light.

Warning
Avoid contact to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face
protection. See Material Safety Data Sheet for additional information.