Tetracycline Hydrochloride - 25g
- For use in cell culture media to inhibit gram-positive and gram-negative bacteria
Tetracycline Hydrochloride is an antibiotic that inhibits protein synthesis (elongation) by preventing the binding of amino-tRNA to ribosome. Tetracycline is for use in cell culture applications.
The tetracycline powder is used to make a stock solution at 5mg/ml in water. The working concentration for stringent plasmids is 10µg/ml and 50µg/ml for relaxed plasmids.
If dissolved in ethanol, the antibiotic solution need not be sterilized.
Magnesium ions are antagonists of tetracycline. Use media without magnesium salts (e.g. LB medium) for the selection of bacteria resistant to tetracycline.
Molecular Formula: C22H24N2O8HCl
Molecular Weight: 480.90
Potency: Min. 900 μg/mg
pH (1%, water): 2.3 ±0.5
Moisture: Max. 2.0%
4-ephianhydrotetracycline: Max. 2.0%
Molecular Biology Specifications
Antibiotic Inhibition/Sensitivity Assay: Pass