TEMED - 50ml
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TEMED - 50ml

  • $30.11 Model Number IB70120



CAS#: 110-18-9

  • A catalyst used to make polyacrylamide gels for protein electrophoresis and sequencing

TEMED (N,N,N’,N’ – Tetramethylethylene-diamine) is used as a catalyst for polymerization of polyacrylamide gels for protein electrophoresis and sequencing.

Physical Specifications
CAS#: 110-18-9
Molecular Formula: C6H16N2
Molecular Weight: 116.21
Purity: Min. 99.0%

IB70120 - Specification Sheet

Concentration of TEMED in acrylamide gels:

Initiator volume per 10 ml gel solution Initiator final concentration
Ammonium persulfate (10% w/v) 50 µl 0.05%
TEMED (undiluted) 5 µl 0.05%
Initiator volume per 10 ml gel solution Initiator final concentration
Ammonium persulfate (10% w/v) 50 µl 0.05%
TEMED (undiluted) 10 µl 0.1%
Initiator volume per 10 ml gel solution Initiator final concentration
Riboflavin (0.1% w/v) 50 µl 0.0005% (5 µg/ml)
Ammonium persulfate (10% w/v) 15 µl 0.015%
TEMED (undiluted) 15 µl 0.05%

Why won’t my acrylamide gel polymerize? It could be one of these three problems: 1. Not enough TEMED in the acrylamide solution 2. If the TEMED is old, the will not polymerize 3. If a gel doesn’t polymerize, the problem is probably the TEMED