Recombinant Chicken Protein IL-16


Recombinant Chicken Protein IL-16

Model Number RPC-160


  • Item Description
  • Specifications
  • Technical Information
  • Predicted molecular weight:12.6 kDa
  • No endotoxins, TAGS, and carrier-free
  • Naturally folded and post-translationally modified

IBI recombinant proteins are expressed inPichia pastoris(yeast) system which provides many advantages overE. colisystems. Yeast systems promote proper protein folding and post-translational modifications for greater bio-activity. The recombinant proteins are endotoxin free and are purified without HIS-TAGS or any other TAG. IBI’s yeast expression system has proven to produce more active proteins that more closely resemble their host form.

Interleukin 16 (IL-16) is a pleiotropic cytokine that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4, and has effects on mixed lymphocyte reaction and inhibition of HIV viral replication. Because this cytokine was discovered in 1982 for its T lymphocyte chemotactic activity, it was named “Lymphocyte Chemoattractant Factor (LCF)” until it was designated interleukin-16. IL-16 forms homotetramers and this sturucture is required for its bioactivity. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. IL-16 is released by a variety of immune (T cells, esinophils, and dendritic cells) and non-immune (fibroblasts, epithelial, and neurona) cells. In the non-diseased state, IL-16 mRNA is almost exclusively expressed on lymphatic tissue, and high levels in T cells. During inflammation, IL-16 is synthesized in a number of other tissues. The sequence, structure, and function of IL-16 are highly conserved in all species examined.Thus far, all species of IL-16 tested induce similar bioactivities on CD4+ T cells.

The recombinant chicken protein IL-16 has a predicted molecular weight of 12.6 kDa.

All IBI recombinant proteins are lyophilized and shipped at room temperature without carrier protein. Proteins can be reconstituted in sterile PBS with at least 0.1% of carrier protein such as BSA or cell assay media.

The IL-16 chicken protein can be used in cell culture, as an IL-16 ELISA Standard, and as a Western Blot Control.

Country of Origin: USA

Formulation/Reconstitution:Lyophilized, Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
Molecular Weight:12.6 kDa (calculated)
Purity:>95% (as visualized by SDS-PAGE analysis)
Purification:Ion-exchange chromatography
Entrez Gene ID:374270

Recombinant Chicken Protein IL-16 - Specification Sheet

How do I reconstitute the recombinant protein?

--Most of the recombinant proteins can be reconstituted in sterile phosphate-buffered saline with 0.1% carrier protein, which is most commonly Bovine Serum Albumin (BSA) --Recombinant bovine proteins cannot be reconstituted in BSA because BSA could cause a reaction in cell cultures. In the case of bovine proteins, Equine Serum Albumin can be used. --If protein vial is left unopened, it can be stored at room temperature or -20C for up to 12 months. --Once protein is open and reconstituted, it must be stored at -20°C and is viable for up to 3 months. --Avoid repeated freeze/thaw cycles as this could cause denaturation of proteins.

What is the activity of the recombinant protein?

--IBI recombinant proteins are sold in units of micrograms (µg) --The recombinant protein is quantified using the BCA Assay which favors the customer in terms of amount of protein. The BCA assay is not perfect, but at least it is standard for all proteins. --If the activity exists, it will be on the Data Sheet shipped with the product --Activity cannot be determined for the animal recombinant proteins because an assay does not exist. (Only human proteins have an assay to determine activity --If end-user wants to know the activity, they can do an activity titer for each lot they use. This is useful because there can be significant differences in activity from lot to lot. --If customer is concerned, they can buy multiple bottles and store them. Also, we can let customer know if we have a good supply of the lot number they are purchasing if they are going to purchase again in the future.

Can we provide custom proteins?

--We can only make published proteins with a known DNA sequence. --There is a lead time of 6 months for the process from start to finish to be completed. Contact IBI if interested in using custom proteins.

If IBI does not stock the protein I need, what options do I have?

IBI may carry a protein that is homologous to the recombinant protein of interest. Because of homology between animals, proteins in a more complicated system are able to cause the same reaction in less complicated systems. For example, chicken proteins can work for amphibian applications.

Why did my protein arrive in multiple vials?

Some proteins are more soluble than others. The proteins that are more difficult to purify can be packaged in multiple vials, so the end-user receives the correct quantity they ordered. Also, lyophilization process works best with volumes of 100 µg or less.


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