Ponceau S
Model Number IB01100
Ponceau S protein stain is used to visualize proteins on a nitrocellulose or PVDF membrane. To create a Ponceau S solution, dissolve 0.5g of Ponceau S in 1ml of glacial acetic acid and bring to 100ml with ddi water.
- Item Description
- Physical Specifications
- Certificate of Analysis
- SDS
- Technical Information
Additional Item Description:
To stain with Ponceau S, remove blot and rinse with ddi water. Then, stain with Ponceau S solution for 1 – 2 minutes. Rinse for 3 – 5 minutes with ddi water. Red bands should appear against the white background.
To reversal stain with Ponceau S solution, place the nitrocellulose membrane in Ponceau S solution for 5 minutes at room temperature. Destain in ddi water for 2 minutes and mark the molecular weight standard with indelible ink. To complete the destain, soak in water for 10 minutes. Alternatively, use 0.1M NaOH solution for 5 minutes if proteins of interest are not basic.
Physical Specifications:
CAS#:6226-79-5
Molecular Weight:760.61
Molecular Formula:C22H12N4Na4O13S4
Em (520nm, water):> 27000
Loss on drying %:< 6%
Molecular Biology Specifications:
RNase: None Detected
DNase: None Detected
Protease: None Detected
SDS Link:
Recommended Use:
Ponceau S is a stain used to visualize protein on nitrocellulose or PVDF membranes.
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