Molecular Biology Grade Water – 10 L
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Molecular Biology Grade Water – 10 L

  • $199.42 Model Number IB42140

  • Certified nuclease-free and pyrogen-free
  • 0.1 µm sterile-filtered into certified-clean polycarbonate bottles
  • Steam-sterilized (autoclaved twice)
  • 18.2 Megohm deionized
  • Available in multiple sizes; sold individually and in multi-bottle cases
  • Applications: PCR, RT-PCR, restriction enzyme assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI Molecular Biology Grade, Nuclease Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme assays, modifying enzyme assays, transfection, cloning, transformation and all general molecular biology lab procedures.

IBI Molecular Biology Grade Water is manufactured under stringent conditions. The purification process for this 18.2 megohm, nuclease free product includes continuous deionization, reverse osmosis, UV-treatment, a 0.1 µm filtration, followed by steam sterilization in final packaging.

Our unique water purification process involves sterile-filtration through a 0.1 µm filter- the smallest filter (pore) size available- thus, removing all bacteria, viruses, and pyrogens. Sterility is further maintained by sterile-filtering directly into an aseptic polycarbonate bottle (or container). These FDA-approved polycarbonate bottles are non-leaching and allow for IBI water products to be autoclaved in its final packaging, ensuring terminal sterilization. Unlike PET bottles, IBI polycarbonate bottles can be autoclaved multiple times. If compromised sterility of an open product is a concern, our polycarbonate bottles allow for re-autoclaving to inactivate any contaminating nucleases. Re-autoclaving can be done on the liquid cycle at 121°C/15 psi. (Be sure to slightly disengage the lid during the autoclave cycle.)

Sterility of water is measured by the Sterility Assurance Level (SAL). IBI Molecular Biology Grade Water maintains the highest level of sterility with a SAL=10-6 . Thus, the chance of a living organism being contained in the water is one in a million. In order to maintain sterility, only open this product in a sterile environment, i.e. clean room, tissue culture fume hood, etc.

After manufacturing we test our Water for up to three days. Each lot is individually assayed to ensure that it meets the stated specifications prior to being released from quality control.

IBI can provide Molecular Biology Grade Water in single lot quantities and an array of packaging options from 125 ml sterile bottles to 55 gal. sterile drums. Just email us at and we would be happy to assist you in developing the product that best fits your application.

The document below serves as a specification sheet and certificate of analysis for all Molecular Biology Water products.

Molecular Biology Grade Water Analysis Sheet

Specifications For Molecular Biology Water
Grade Molecular Biology
Cations & Anions Free: Yes
Mycoplasma Free: Yes
Sterility Assurance By: 0.1 µm pore filter
Resistivity: 18.2 mOhms
Endotoxin (LAL): ≤ 0.1 EU/ml
Electrophoresis: Yes
PCR: Yes
MEA Approved: n/a
RNAse: None Detected
DNAse: None Detected
Nickase: None Detected
Protease: None Detected
Phosphatase: None Detected
Mycoplasma: None Detected
Heavy Metals: Not Tested
Iron (Fe): Not Tested
Sterility Assured: 0.1 µm sterile-filtered and 2 X autoclaved
Available Bottle Sizes: 125 ml, 500 ml, 1 L, 2 L, 10 L