Mini Genomic DNA Kit (Plant/Fungi) – 300 Preps
- Sample Size: up to 100mg fresh plant tissue/25mg dry plant tissue
- Expectant Yield: up to 50µg DNA
- Operation Time: 60min or less
- Elution Volume: 50-200µl
- Binding Capacity: 50µg
- Free sample available upon request
The Genomic DNA Mini Kit for Plants and Fungi provide a fast and economical method to isolate total DNA (genomic DNA, mitrochondrial, and chloroplast) from plant tissue and cells. Samples are disrupted both by grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix of the spin column. The contaminants are washed with an ethanol based wash buffer. The genomic DNA is then eluted by a low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. This entire procedure can be completed in 30 minutes or less and the purified genomic DNA is ready for PCR, Real-Time PCR, Southern Blotting, or RFLP.
The quality of the Genomic DNA Mini Kit for Plant is tested on a lot-to-lot basis by isolating genomic DNA from 50 mg young leaf samples. More than 10 µg of genomic DNA is quantified with a spectrophotometer and checked by electrophoresis.
|Specifications||Mini DNA Kit (Plant)|
|Binding Capacity:||50 µg|
|Sample Concentration:||100 mg of fresh plant tissue 25 mg of dry plant tissue|
|Typical Yield:||3-5 µg (100 mg Arabidopsis thaliana leaf) 20-25 µg (100 mg Nicotiana tabacum leaf)|
|Elution Value:||30-200 µl|
|Operation Time:||<30 minutes|
Why do I get low yields when I work with the root? The kit will not work as well with roots because of the high polysaccharide content of the root. Kits IB47230 & IB47231 contain a GPX1 buffer which works better with high polysaccharide samples.
Can this kit be used for fish or mammals? No, the buffers in this kit target the breakdown of components specific to plants. DNA binding may also be affected.
Customer has plant material the texture of sawdust. Does the sample need to be grinded anymore? This texture will work for this kit.
How do you remove excess oil from the plant samples? 1. Cut off 100 mg of fresh plant tissue or 50 mg of dry plant tissue. 2. Freeze the sample with liquid nitrogen. 3. Grind the sample to a fine powder using a mortar and pestle. 4. Add 400 µl of GP1 Buffer or GPX1 Buffer and 5 µl of RNAse A (96-well genomic DNA kit plant) or 1 ml of IBI Plant Isolate and 0.5 µl of RNAse A (IBI Plant Isolate kit) to the sample in the mortar. 5. Continue grinding the sample until it is completely dissolved then transfer the sample lysate to a 1.5 ml micro-centrifuge tube. 6. Incubate the sample lysate at 65°C for 10-30 minutes then centrifuge at 14-16,000 x g for 5 minutes. 7. A layer of oil will float on the top; transfer the “clear supernatant” only to a clean 1.5 ml micro-centrifuge tube then follow the protocol.
How small of a viral DNA fragment can be extracted from a plant sample using the Mini Genomic DNA Plant Kit? It can extract fragments as small as 100 bp from virus infected plants.