MINI FlexTube Kit, 25KDa MWCO - 30 Pack
- MWCO: 25KDa
- FlexTube Volume: 250µl
- Dialysis Volume: 10-250µl
- Minimum Sample Size for Extraction: 0.5µg
- Maximum Gel Slice: 0.4cm x 1.1cm
IBI MINI FlexTubes are typically used for the following applications: dialysis, gel extraction, electro-elution, and de-salting. The design of the FlexTube allows for easy loading and unloading with a standard pipette tip and screw-cap top. Multiple tubes can be placed in one buffer tank for high-throughput applications with minimal hands-on time.
The FlexTube membrane is constructed of regenerated cellulose and is 18 - 42µm thick. The membrane dimensions for the MINI FlexTube is 0.4 x 1.1cm.
For the MINI FlexTube, the minimum sample size is 0.5µg, the maximum gel slice is 0.4cm x 1.1cm, and the dialysis volume is 10-250µl. The MINI Flextube is offered with the following Molecular Weight Cut-Offs (MWCO): 6-8KDa, 12-14KDa, or 25KDa.
When used for dialysis or buffer exchange, the MINI FlexTubes can be used with DNA, RNA, proteins, and Oligonucleotides (>15nt) for small volumes of 10-250µl. Simply load the sample into the tube with a standard pipette, load into the FlexTube float, and place in a stirred beaker of desired buffer.
When used for gel extraction, the FlexTubes can be used with proteins, RNA, DNA, or oligonucleotides (>20nt) from polyacrylamide, agarose, or any other gel matrix in a running buffer. The tubes are ideal for protein-nucleic acid complexes and large size DNA fragments (>100Kb) from agarose or polyacrylamide gels. DNA recovery from polyacrylamide or agarose gels is typically greater than 90%. See manual for more approximate times.
Isolation of proteins from a gel using the FlexTube delivers up to 70% recovery yield. After the protein gel extraction, the sample can remain in the tube for SDS removal*. The combination of SDS-PAGE, electro-elution with the FlexTube, and SDS removal buffer can give you a more accurate determination of a protein’s molecular mass due to the high recovery yield and purity of the sample. This also results in a MALDI-MS analysis with greater sensitivity**.
The same FlexTube can also be used for Oligo PAGE Electro-Elution and De-Salting. This method is a vast improvement over the Diffusion Method, which is a lengthy over-night process that typically produces low yields. The FlexTube will allow the extraction of the protein from the gel followed by de-salting of the sample by dialysis in the same FlexTube. The easy-to-follow protocol will save you valuable lab time.
IBI MINI FlexTubes are tested by using them in the extraction of proteins, RNA, or DNA fragments of various sizes from either agarose or polyacrylamide gels. IBI MINI FlexTube kits are also tested for simple dialysis of salts or buffer exchanges. The quality of the isolated protein, RNA, or DNA fragments, or of the sample after dialysis, is checked by several assays commonly used for proteins, nucleic acids, and dialysis.
The FlexTube is a single-use product.
*To use the FlexTubes for protein gel extraction, SeeBand Protein Stain (Extraction Grade) must be used. Coomassie blue fixes the proteins in the gel, whereas, SeeBand Protein Stain does not. Therefore, SeeBand Protein Stain is necessary for protein gel extraction. SeeBand Protein Stain is an environmentally friendly stain with high sensitivity and does not contain methanol or acetic acid. SeeBand Protein Stain does not require use of hazardous solvents for destaining.
Molecular Weight Cut-Off (MWCO): 25KDa
FlexTube Volume: 250µl
Dialysis Volume: 10-250µl
Minimum Sample Size for Extraction: 0.5µg
Maximum Gel Slice: 0.4cm x 1.1cm
Yield of Molecule Recovery: 90% from an agarose or polyacrylamide gel; 70% from SDS-PAGE gel
Membrane: Ultra-clean, sulfur- and heavy metal-free. EDTA-treated.
Why do my FlexTubes have water in them and in the bag they came in? Some FlexTubes are shipped with water inside of the tube to protect the membrane. Some water might leak through the membrane and water is added to the bag as well.
Can I tape another tube to the electro-elution tray to run more FlexTubes at one time? Yes, as long as the arrow on the tube is facing up, and the membranes are facing the electric field.
What Molecular Weight Cut Off do I need? Anything below the listed MWCO can travel through the membrane. To calculate the MW of a nucleic acid: 330 Da MW of 1 base 660 Da MW of 1 base pair
What type of stain do I need if I want to electro-elute protein from an acrylamide gel? SeeBand Stain (Extraction) grade is needed because it won’t fix the proteins in the gel. Stains such as Coomassie Blue will fix the proteins in the gel and the proteins will not come out during electro-elution.
Can I concentrate my sample in the Flextube by centrifugation? No, if the Flextube is centrifuged, the fluid will leak out of the membrane.
After electro-elution, can I use the same tube for dialysis of my sample? Yes, remove gel from tube and then dialyze against a large amount of water. However, some gel residue may still be in the sample from electro-elution. Refer to the Oligo protocol for more information.