Midi Fast Ion Plasmid Kit Endotoxin-Free - 25 Preps
- Sample Size: 50ml high-copy plasmid/100ml low-copy plasmid
- Operation: Gravity Flow Column
- Expectant Yield: 200µg of plasmid DNA
- Operation Time: 120 minutes or less
FREE UPS Ground Shipping!!
The Midi Plasmid Kits Endotoxin Free use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than two hours. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.
The quality of the Fast Ion Midi Kits is tested on a lot-to-lot basis by isolating plasmid DNA from a 50 ml overnight E. coli (DH5α) culture, containing pBluescript (A600>2U/ml). More than 120 µg of plasmid DNA is quantified with a spectrophotometer. The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.
|Specifications||Midi Fast Ion Plasmid Kit (Endotoxin-Free)|
|Binding Capacity:||800 µg|
|Sample Concentration:||50-300 ml|
|Sample Type:||cultured bacterial cells (high-copy = 50-200 ml, low-copy = 100-300 ml)|
|Fragment Size:||1-20 kb|
|Typical Yield:||200-800 µg|
|Elution Volume:||500 µl- 2 ml|
|Operation Time:||<120 minutes|
What competitor’s kit is comparable? QIAGEN EndoFree Plasmid Maxi Kit
What is the formulation for the PW buffer? This is not exact; it is QIAGAN’s formulation which will work fine. 1.0 M NaCl, 50 mM MOPS pH 7.0 15% Isopropanol.