Methylene Blue – 25g
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Methylene Blue – 25g

  • $28.43 Model Number IB74050



CAS# 7220-79-3

  • Used to stain agarose gels when recovery of DNA is required

Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA.

To stain DNA, stain a 1% gel for 15 min with 0.02% methylene blue solution in distilled water. Destain with distilled water for 15 minutes.

To stain RNA bonded to nylon or nitrocellulose membranes, soak the dried membrane in 5% acetic acid at room temperature for 15 minutes. Then, place the filter in 0.5 M sodium acetate (pH 5.2) and 0.04% methylene blue for 5 – 10 minutes at room temperature. To destain, rinse the filter in water for 5 – 10 minutes.

Physical Specifications
CAS#: 7220-79-3
Formula Weight: 373.9
Molecular Formula: C16H18CIN3S x 3(H2O)
Em (661 nm, water): >69,000
Arsenic: 0.0008%
Copper: <0.01%
Moisture Max.: 15.0%
Zinc: <0.001%
Lead: <0.001%
Dye Content (Dry Basis): 98 - 103% (The Dye Content can be considered a purity specification)

IB74050 - Specification Sheet

Recommended Use
Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using Methylene Blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA.
1. Stain a 1% gel for 15 minutes with 0.02% Methylene Blue Solution in distilled water.
2. Destain with distilled water for 15 minutes.

Used for staining RNA that is bonded to nylon and nitrocellulose membranes.
1. Soak the dried filter in 5% acetic acid at room temperature for 15 minutes.
2. Place the filter in 0.5 M Sodium Acetate (pH 5.2) and 0.04% Methylene Blue for 5-10 minutes at room temperature.
3. Rinse the filter in water for 5-10 minutes.

Purity of Methylene Blue: The purity of methylene blue is stated as Dye Content as measured on a dry basis. The Dye Contenet for IBI Methylene BLue is 98% - 103%.