MaGELin Mini Protein Start-Up Kit
The IB94000B MaGELin Mini protein system Start-up kit comes with additional items you need to pour 10 cm x 10 cm cast-your-own gels including, 1 liter 10X TRIS-Glycine-SDS buffer, 5ml of 2X Protein Loading Dye, and a 1 liter bottle of Blue Clean Protein stain. This highly versatile system will also accommodate the IB95000 Western Transfer module so when you’re not running gels the unit can be used as a dedicated Western transfer system. The IB94000 comes complete with:
- Dual gel capture device for running two gels simultaneously.
- Dual gel casting system that allows casting outside of the running unit.
- Three sets of glass plates for pouring and running gels
- Two sets of special 0.8 mm side spacers to seal the gel sandwich during pouring
- Buffer tank, vented lid, power cords
- Two 0.8 mm x 12-tooth combs.
- 10X TRIS-GLYCINE-SDS buffer, 2X Protein loading dye, Blue Clean Protein stain
The IB94000 utilizes a unique spacer and casting system to make it easy and fast to cast your own gels. The special side spacers are made specifically to accommodate the thickness of each glass slide that comes with the unit. These side spacers will produce a 0.8 mm thick gel. Once the glass plates have been assembled using the side spacers, they can now be inserted into the gel casting unit. This unit accommodates two sets of glass plates simultaneously. The bottom of the gel casting unit contains a very soft gel-like gasket that will seal the bottom of the gel sandwich. Once the gel sandwich is placed inside the casting unit, place the lid of the casting unit on top and press gently to fasten using the included thumb screws. Once fastened the lid will provide the correct amount of down pressure on the gel sandwich to seal the bottom and hold them in place. The polyacrylamide solution can now be introduced through the opening in the casting fixture lid.
Unit dimensions: 18.0 cm(H) x 17.0 cm(W) x 17 cm(L)
Gel dimensions: 10.0 cm(W) x 10 cm(L)
Max sample capacity: 15 samples
Buffer capacity: 350 ml min. – 850 ml max.
Max volt: 150 V
General Information about Vertical Electrophoresis Our vertical electrophoresis unit “spacers” are customized for our units only. They cannot be used on another company’s unit. Our spacers are orange rubber pieces in which the glass fits into and is held in place.
Options for Cooling a Vertical Gel System 1. The user can make up buffer ice cubes (use same buffer that they are running their gel in.) These can be placed in the buffer tank while running the gel to cool it. 2.For the MaGELin systems, an ice pack can be purchased in placed in the buffer tank to cool it down. The product# is IB92080.
Which pre-cast gels can be used for this system? 1. (Fisher’s) Expedeon Gels—These are the PAGE gels (little gels). 2. Invitrogen (Life Technologies) gels— NuPAGE gels or Novex.